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https://www.nature.com/articles/s41598-018-38119-9
Feb 15, 2019 · Although Cao et al. demonstrated the delivery of a modified Cre protein into rice calli after protoplasting or plasmolyzing and achieved regeneration of rice plants with Cre-excised …Author: Yuichi Furuhata, Ayako Sakai, Tomi Murakami, Mone Morikawa, Mone Morikawa, Chikashi Nakamura, Chikas...
https://www.takarabio.com/learning-centers/gene-function/gene-editing/cre-recombinase/fast-cre-delivery-with-gesicle-technology
Cre Recombinase Gesicles are cell-derived nanovesicles that deliver active Cre recombinase protein directly to your target cells. Simpler to use than either plasmid or viral gene delivery, these gesicles allow you to quickly and efficiently flox a broad range of cell types on demand.
https://www.pnas.org/content/113/11/2868
Mar 15, 2016 · The delivery of supercharged Cre protein and Cas9:sgRNA complexed with bioreducible lipids into cultured human cells enables gene recombination and genome editing with efficiencies greater than 70%. In addition, we demonstrate that these lipids are effective for functional protein delivery into mouse brain for gene recombination in vivo.Author: Ming Wang, John Zuris, Fantao Meng, Holly Anne Rees, Shuo Sun, Pu Deng, Yong Han, Xue Gao, Dimitra P...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289409/
Delivery of Cre and TALE proteins was performed by combining 1 nM to 1 µM protein (based on a 275 µL final volume) with 0.2–2.5 µL commercially available cationic lipids in 25 µL OPTIMEM media (Life Technologies) according to the manufacturer’s protocol for normal plasmid transfection, including incubation time. 25 µL of the OPTIMEM mixture containing cationic lipids and protein was then added …Author: John A. Zuris, John A. Zuris, David B. Thompson, David B. Thompson, Yilai Shu, John P. Guilinger, Jo...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171028/
Jul 01, 2014 · Delivery of toxic amounts of Cre to the nucleus was only observed when transfecting the NLS-Cre encoding plasmid, which allows amplification of NLS-Cre by protein expression and active transport to the nucleus due to the NLS.Author: Andrea Lj Marschall, Congcong Zhang, André Frenzel, Thomas Schirrmann, Michael Hust, Franck Perez, S...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3193192/
Oct 11, 2011 · Cre recombinase delivery using a cell-penetrating peptide fusion (HIV TAT) and VLPs. (A) Activation of RFP expression following the action of Cre in a reporter cell line containing a stably integrated expression cassette (Lox1-GFP-Lox2-RFP). (B) A reporter ...Author: Stanislaw J. Kaczmarczyk, Kalavathy Sitaraman, Howard A. Young, Stephen H. Hughes, Deb K. Chatterjee
https://www.cell.com/cell/fulltext/S0092-8674(15)00315-3
Apr 23, 2015 · Protein manipulation is usually achieved indirectly, at the DNA or RNA level, either by “knockdown” or mutation of the encoding gene or by ectopic overexpression of wild-type or mutant genes. Transient, non-integrative modulation of cell function by direct intracellular delivery of proteins has appealing application,...Author: Diego S. D’Astolfo, Romina J. Pagliero, Anita Pras, Wouter R. Karthaus, Hans Clevers, Vikram Prasad,...
https://www.nature.com/articles/nchem.2779
May 22, 2017 · The fidelity of this approach was confirmed through the intracellular delivery of a ribosome-inactivation protein (saporin), Cre recombinase and IgG delivery, which resulted in a specific labelling...Author: Misao Akishiba, Toshihide Takeuchi, Yoshimasa Kawaguchi, Kentarou Sakamoto, Hao-Hsin Yu, Ikuhiko Nak...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3505079/
For Cre protein fusion delivery analysis: incubate cells for 24 to 48 hours post-treatment to allow reporter signal to maximize. Cells should be analyzed for reporter protein expression as appropriate.Author: David B. Thompson, James Cronican, David Ruchien Liu
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