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https://www.sciencedirect.com/science/article/pii/S0168365902003693
Jan 09, 2003 · Controllable delivery of non-viral DNA from porous scaffolds. ... The inductive approach to tissue engineering combines three-dimensional porous scaffolds with drug delivery to direct the action of progenitor cells into a functional tissue. We present an approach to fabricate scaffolds capable of controlled, sustained delivery by the assembly ...Author: Jae Hyung Jang, Lonnie D. Shea
https://www.researchgate.net/publication/10984414_Controllable_delivery_of_non-viral_DNA_from_porous_scaffolds
Controllable delivery of non-viral DNA from porous scaffolds Article in Journal of Controlled Release 86(1):157-68 · February 2003 with 9 Reads How we measure 'reads'
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2657198/
DNA can be delivered alone (i.e., plasmid), or can be packaged using viral or non-viral vectors. Viral vectors provide greater efficiency than either naked plasmid or non-viral vectors, yet provoke an immune response that can lead to clearance of the vector, and cells expressing the transgene.Author: David M. Salvay, Lonnie D. Shea
https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(05)00181-4
In summary, highly porous PLG scaffolds releasing plasmid DNA induced prolonged in vivo transgene expression up to 105 days, which was sufficient to promote physiological responses. The transgene expression was localized within the scaffold and adjacent to polymer, suggesting that the surface areas may affect transgene expression.Author: Jae Hyung Jang, Christopher B. Rives, Lonnie D. Shea
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2648405/
Plasmid Delivery in Vivo from Porous Tissue-Engineering Scaffolds: Transgene Expression and Cellular Transfection Jae-Hyung Jang , 1 Christopher B. Rives , 1 and Lonnie D. Shea 1, 2, * 1 Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road E156, Evanston, IL 60208-3120, USAAuthor: Jae Hyung Jang, Christopher B. Rives, Lonnie D. Shea
https://www.nature.com/articles/gt201079
May 20, 2010 · Biomaterial scaffolds that serve as vehicles for gene delivery to promote expression of inductive factors have numerous regenerative medicine applications. In …Author: David M. Salvay, Marina Zelivyanskaya, Lonnie D. Shea
https://www.researchgate.net/publication/7791204_Plasmid_Delivery_in_Vivo_from_Porous_Tissue-Engineering_Scaffolds_Transgene_Expression_and_Cellular_Transfection
Plasmid Delivery in Vivo from Porous Tissue-Engineering Scaffolds: Transgene Expression and Cellular Transfection. ... Controllable delivery of non-viral DNA from porous scaffolds. J. Controlled.
https://www.nature.com/articles/3302918
Feb 22, 2007 · Collagen scaffolds for nonviral IGF-1 gene delivery in articular cartilage tissue engineering ... Type II collagen-GAG scaffolds. Porous ... Controllable delivery of …Author: R M Capito, R M Capito, M Spector, M Spector
http://europepmc.org/articles/PMC2927809
In this report, we investigate gene transfer in vivo using surface immobilization of naked plasmid to porous PLG scaffolds. Porous PLG scaffolds have been widely employed for numerous applications in regenerative medicine, and delivery of gene therapy vectors has the potential to enhance their bioactivity.
https://academic.oup.com/nar/article/33/19/e170/1308860
Jan 01, 2005 · Recently, new biomaterials, such as the gene activated matrix (GAM) and biodegradable polymers (i.e. polylactide-co-glycolide, PLGA) have been developed that can serve as scaffolds for DNA delivery and tissue engineering (2,3) Even though non-viral gene delivery systems offer enhanced safety over viral systems, they are plagued with ...Author: Dehai Liang, Yen K. Luu, Kwangsok Kim, Benjamin S. Hsiao, Michael Hadjiargyrou, Benjamin Chu
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