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https://www.sciencedirect.com/science/article/pii/S0001868618303865
Chemical modification could improve the performance of chitosan in gene delivery systems. • Various ligands could be utilized to promote the targetability of chitosan. • Chitosan can be used in gene vectors with different functions. • Carboxymethyl chitosan and PEGylated chitosan have been used in CRISPR/Cas9 system delivery.Author: Di Chuan, Tao Jin, Rangrang Fan, Liangxue Zhou, Gang Guo
https://www.sciencedirect.com/science/article/pii/S0939641112001038
As chitosan is comparatively less toxic than other cationic polymers such as polyethyleneimine, polylysine, or polyarginine , it is therefore a promising excipient for non-viral gene delivery systems. In addition, chitosan–DNA-based drug complexes protect at least to some extend towards degradation by DNAses in this way improving the ...Author: Andreas Bernkop-Schnürch, Sarah Dünnhaupt
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4960152/
Chitosan which is a cationic polymer was used as gene delivery system. Stable HEK-293 cell lines were established by transfection of cells with complexes which were prepared with chitosan and pVitro-2 plasmid vector that contains neomycin drug resistance gene, beta gal and GFP genes.Author: Emine Şalva, Suna Özbaş Turan, Ceyda Ekentok, Jülide Akbuğa
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6627531/
Jun 25, 2019 · Thus, some working deliveries can be seen showing promising gene silencing/transfection effects in vivo in recent works. Although extensive research has been done on chitosan-based gene delivery vectors, clinical translation of chitosan-based delivery system has not been reported yet.Author: Ye Cao, Yang Fei Tan, Yee Shan Wong, Melvin Wen Jie Liew, Subbu Venkatraman
http://www.nature.com/articles/3301492
Jul 13, 2001 · Chitosan is a natural cationic linear polymer that has recently emerged as an alternative nonviral gene delivery system. We have established the relationships between the structure and the ...Author: Magnus Köping-Höggård, Ioannis Tubulekas, Holly Guan, Katarina Edwards, Mats Nilsson, Kjell Mårten V...
https://www.researchgate.net/publication/299975169_Chitosan_Gene_Delivery
Gene delivery is the most innovative therapeutic method in the fi eld of molecular medicine. Viral and nonviral vectors, which are the carrier systems for the gene transfer, are used for the gene ...
https://www.researchgate.net/publication/298818169_Chitosan_Nanoparticles_in_Gene_Delivery
An efficient gene delivery system for cellular uptake of DNA is essential for gene therapy. Chitosan has been widely used as a promising non-viral carrier for gene delivery.
https://link.springer.com/chapter/10.1007%2F978-3-662-47862-2_22
An efficient gene delivery system for cellular uptake of DNA is essential for gene therapy. Chitosan has been widely used as a promising non-viral carrier for gene delivery. It is a cationic polymer with free amino groups and can bind to anionic nucleic acids spontaneously.Author: Julide Akbuga, Suna Ozbas-Turan, Ceyda Ekentok
https://pubs.acs.org/doi/abs/10.1021/acs.molpharmaceut.8b00434
Chitosan has been widely employed to deliver nucleic acids such as siRNA and plasmids. However, chitosan-mediated delivery of a gene-editing system has not been reported yet.Author: Hairui Zhang, Tania F. Bahamondez-Canas, Yajie Zhang, Jasmim Leal, Hugh D.C. Smyth
https://pubs.acs.org/doi/abs/10.1021/bc0501597
Dec 27, 2005 · PEI-g-chitosan of Mn = 7400 with a polydispersity index (PDI) of 1.50, and PEI side chains of Mn = 206 was prepared for gene delivery. Gel electrophoresis showed that DNA migration was retarded completely at a N/P ratio of 2.5/1, indicating good DNA …Author: Kokhou Wong, Guobin Sun, Xueqing Zhang, Hui Dai, Ye Liu, Chaobin He, Kam W. Leong
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