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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3787071/
Jun 15, 2010 · Non-toxic lipid-coated nano-calcium-phosphate particles exhibiting high DNA transfection efficiency were prepared by a new and simple method. Efficient delivery of plasmid DNA was achieved in a mammalian cell culture using LNCP/pDNA complexes. By incorporating a calcium-phosphate core, the LNCP was able to deliver DNA very efficiently.Author: Chenguang Zhou, Bo Yu, Xiaojuan Yang, Tianyao Huo, L. James Lee, Rolf F. Barth, Robert J. Lee
https://www.ncbi.nlm.nih.gov/pubmed/27213045
Sep 07, 2015 · Preparation of optimized lipid-coated calcium phosphate nanoparticles for enhanced in vitro gene delivery to breast cancer cells. Tang J(1), Li L(1), Howard CB(1), Mahler SM(2), Huang L(3), Xu ZP(1). Author information: (1)Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD 4072, Australia.Author: Jie Tang, Li Li, Christopher B. Howard, Stephen M. Mahler, Leaf Huang, Zhi Ping Xu
https://www.sciencedirect.com/science/article/pii/S0168365910009168
Calcium phosphate-DNA co-precipitates have been used for gene delivery for more than 35 years and have the advantage of being nontoxic, easy to produce, and having the ability to complex nucleic acids leading to efficient transfection. Conventional synthetic methods yield particles that are only stable for a short period of time.Author: Elisabeth V. Giger, Josep Puigmartí-Luis, Rahel Schlatter, Bastien Castagner, Petra S. Dittrich, Jea...
https://www.sciencedirect.com/science/article/pii/S037851730400540X
We presume that these calcium phosphate nanoparticles have well-defined role in DNA delivery so far as endosomal escape, protection against nuclease degradation and nuclear uptake are concerned and, therefore, can be used as an effective non-viral vector in gene therapy.Author: Savita Bisht, Gajadhar Bhakta, Susmita Mitra, Amarnath Maitra
https://www.intechopen.com/books/hydroxyapatite-advances-in-composite-nanomaterials-biomedical-applications-and-its-technological-facets/gene-delivery-by-hydroxyapatite-and-calcium-phosphate-nanoparticles-a-review-of-novel-and-recent-app
Gene therapy is a targeted therapy which can be used in the treatment of various acquired and inherited diseases. Inhabitation of a gene function, restoring or improving a gene, or gaining a new function can be achieved by gene therapy strategies. The most crucial step in this therapy is delivering the therapeutic material to the target. Nanosized calcium phosphates (CaPs) have been considered ...Author: Feray Bakan
https://www.researchgate.net/publication/286971741_Calcium_Phosphate_System_for_Gene_Delivery_Historical_Background_and_Emerging_Opportunities
Calcium phosphate system has been used widely in in vitro gene delivery for almost four decades. Excellent biocompatibility and simple application have motivated the researchers to always consider ...
https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/transfection-basics/gene-delivery-technologies.html
Selecting a Viral DNA Delivery System › Transfection Methods › Gene Delivery Technologies Cationic Lipid Transfection Calcium Phosphate Transfection DEAE-Dextran Transfection Other Cationic Polymers Viral Transfection Electroporation
https://www.thermofisher.com/in/en/home/references/gibco-cell-culture-basics/transfection-basics/gene-delivery-technologies/calcium-phosphate-co-precipitation.html
Calcium phosphate co-precipitation has been a popular transfection method since its introduction in the early 1970s (Graham and van der Eb, 1973) because the components it …
https://www.researchgate.net/publication/259488402_A_Mini_Review_of_Biodegradable_Calcium_Phosphate_Nanoparticles_For_Gene_Delivery
In this review, we focus on the current research activity in the development of calcium phosphate nanoparticlss for gene delivery. Calcium phosphate nanoparticles are mainly classified into lipid ...
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