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2020-10-13 · After a total of 6 hours, samples were kept < 4°C and stained with Live/Dead Aqua and anti-CD16/CD32 blockade as above, fixed and permeabilized using the Cytofix/Cytoperm kit (BD Biosciences #554714), and stained intracellularly for 1 hour in 0.1% BSA in PBS with antibodies including (each at 2 μg/ml): anti-CD3 APC-Cy7 (BD Biosciences #557596 ...Location: 8600 Rockville Pike, Bethesda, MD
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