Biolistic Pds-1000/He Particle Delivery System Demonstration

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Gene Gun demonstration on how to make GMOs - YouTube

    https://www.youtube.com/watch?v=57E55EKcUgA
    Nov 05, 2015 · A gene gun or a biolistic particle delivery system, originally designed for plant transformation, is a device for injecting cells with genetic information. The payload is an elemental particle of ...Author: Marlo Asis

PDS-1000/He

    http://www.biorad-ads.com/09-0804_educational_tools/pds/pds_faq.htm
    FAQ's : What grade of helium is used with the PDS1000/He? How is the Biolistic Purchase/Demonstration Agreement processed? What is the Upper size range of DNA that can be coated onto particles for Biolistic Gene Gun delivery? What's the difference between the Helios Gene Gun and the PDS-1000He? Q. What grade of helium is used with the PDS1000/He?

Miscellaneous - Seed Biotechnology Center

    http://sbc.ucdavis.edu/Useful_Websites/Miscellaneous/
    StatLIA Demonstration Guide (commercial software package) HHMI's BioInteractive: Virtual Labs HHMI Virtual Lab (ELISA) Plant Transformation CEPRAP - Plant Transformation BioRad Biolistic PDS-1000/He Particle Delivery System (Bulletin_1700.pdf) Biolistics - Prof. Ruoff Biolistic Transformation Methods & Applications of Plant Cell & Tissue Culture

Proteolistics: A biolistic method for intracellular ...

    https://www.researchgate.net/publication/264502458_Proteolistics_A_biolistic_method_for_intracellular_delivery_of_proteins
    In this work, an intracellular protein delivery methodology termed "proteolistics" is described. This method utilizes a biolistic gun apparatus and involves a simple protein/projectile preparation ...

Transformation of the Diatom Phaeodactylum Tricornutum ...

    https://www.researchgate.net/publication/227878022_Transformation_of_the_Diatom_Phaeodactylum_Tricornutum_Bacillariophyceae_with_a_Variety_of_Selectable_Marker_and_Reporter_Genes
    Nuclear transformation of the knockout mutants PtAUREO1a KO8 and KO9 of P. tricornutum was performed using a Bio-Rad Biolistic PDS-1000/He Particle Delivery System (Bio-Rad, Hercules, CA, USA ...

Schistosoma mansoni miracidia transformed by particle ...

    https://www.sciencedirect.com/science/article/pii/S0014489403001681
    For particle bombardment (biolistics), approximately 500 miracidia or 20 male, adult worms were evenly spread onto a polycarbonate membrane (Transwell, Costar), water or medium was removed and target schistosomes on the polycarbonate membranes were positioned in a Biolistic PDS-1000/HE Particle Delivery System (Bio-Rad Laboratories GmbH ...Author: Oliver Heyers, Anna K. Walduck, Paul J. Brindley, Wilfrid Bleiss, Richard Lucius, Tomislav Dorbic, B...

JMJ27, an Arabidopsis H3K9 histone demethylase, modulates ...

    https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.13623
    Jun 26, 2017 · A transient expression assay was performed in which this construct was delivered into onion epidermal cells using a Biolistic PDS‐1000/He Particle Delivery System. Empty vector (35S::GFP, modified from pMDC83; Curtis and Grossniklaus, 2003) was used as a control. The transformed epidermal peels were stained for 4′,6‐diamidino‐2 ...Author: Aditya Dutta, Pratibha Choudhary, Julie Caruana, Ramesh Raina

Three ethylene‐responsive transcription factors in tobacco ...

    https://onlinelibrary.wiley.com/doi/full/10.1046/j.1365-313x.2000.00709.x
    Biolistic particle bombardment. Particle bombardments were carried out with the Biolistic PDS‐1000/He Particle Delivery system (Bio‐Rad). An amount of 0.8 μg of plasmids were coated to gold microcarriers (500 μg per bombardment; 1.0 μm particle size) by a standard method ( Sanford et al. 1993 ). The microcarriers were bombarded onto ...Author: Masaru Ohta, Masaru Ohme-Takagi, Hideaki Shinshi

OXS2 is Required for Salt Tolerance Mainly through ...

    https://www.nature.com/articles/s41598-019-56456-1
    Dec 30, 2019 · Transient expression in onion epidermal cells was carried out using the Biolistic PDS 1000/He Particle Delivery System (Bio-Rad) (1100 psi, 10 cm traveling distance) with plasmid DNA, 0.5 M …Author: Ying Jing, Lin Shi, Xin Li, Han Zheng, Jianwei Gao, Mei Wang, Lilong He, Wei Zhang

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