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https://www.youtube.com/watch?v=dfD95gsEdrg
Nov 03, 2015 · This video provides an overview of the PDS-1000 / He™ and Hepta™ Biolistic Particle Transfection Systems, including instrument setup, consumable preparation, instrument operation and shut down.Author: Bio-Rad Laboratories
https://msu.edu/course/css/451/Lecture/WK2-PT-Biolistic(2009).pdf
fAdvantage: yThis method can be use to transform all plant species. yNo binary vector is required. yTransformation protocol is relatively simple. fDisadvantage: yDifficulty in obtaining single copy transgenic events. yHigh cost of the equipment and microcarriers. yIntracellular target is random (cytoplasm, nucleus, vacuole, plastid, etc.). yTransfer DNA is not protected.
http://www.bio-medicine.org/biology-technology/Optimization-of-Biolistic-Transformation-Using-the-Helium-Driven-PDS-1000-He-System-1199-1/
back to top microparticle and particle accelerator parameters with Bio-Rad's PDS-1000/He system using two biological systems, transient expression of the -glucuronidase (GUS) gene in cauliflower epidermal cortex, and stable expression of a wild-type URA 3 gene in an auxotrophic mutant of Saccharomyces cerevisiae. The microparticle variables ...
http://www.bio-thing.com/p120983
Biolistic PDS-1000/He Particle Delivery System ... Bio-Rad Laboratories: Catalog#: 165-2255LEASE. Similar Protocol. Delivery of the Cas9 or TevCas9 System into Phaeodactylum tricornutum via Conjugation of Plasmids from a Bacterial Donor
http://www.biotech.iastate.edu/ptf/maize-genetic-transformation-biolistic-mediated/
Immature B104 embryos are bombarded using a Bio-Rad Biolistic PDS-1000/He apparatus and plasmid provided by the client. On average, three* independent bialaphos-resistant events represented by multiple clonal plantlets are delivered to the client at the completion of the project.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC355893/
The Bio Rad PDS 1000/He system is a helium burst that uses a macrocarrier system device. The macrocarrier consists of a floppy, thin kapton disk (45 µm in thickness) that is accelerated by a compressed helium burst depending on the value of a rupture disk.
https://www.somatco.com/PDS-1000-he-hepa-system.pdf
GENE TRANSFER: BIOLISTICS Biolistic PDS-1000/He™ System Applicable to a wider range of biological targets than any other transformation method Bypasses membrane receptor barriers Eliminates the need for potentially toxic biological or chemical vectors Used with the Hepta adaptor, maximizes the number of cells transformed in one bombardment
http://www.biorad-ads.com/09-0804_educational_tools/pds/pds_faq.htm
The Helios Gene Gun is a hand-held instrument which is made for in vivo transfection into animal or plant tissues in a 2cm diameter target area. The PDS-1000He is an in vitro benchtop instrument that has a vacuum sample chamber for more controlled particle delivery into animal or plant cell culture or tissues over a 9cm diameter target area.
https://www.researchgate.net/publication/41421461_Transformation_of_Moss_Physcomitrella_patens_Gametophytes_Using_a_Biolistic_Projectile_Delivery_System
Biolistic particle delivery system (Bio-Rad, PDS-1000/He) B efore use, clean the chamber with H 2 O followed by 100% ethanol and allow it to dry. Incubator preset to 25ºC, with white light at ...
https://www.sciencedirect.com/science/article/pii/S0167701202001264
Effective biolistic transformation of intact conidia from the filamentous fungus Trichoderma reesei was achieved using the Bio-Rad Hepta Adaptor system with seven barrels for particle launch. Transformation frequencies of up to 39 colonies per μg of circular DNA and 37 colonies per μg of linear DNA were obtained at an optimal target distance of 3 cm and a helium pressure of 1350 psi.
https://www.nature.com/articles/nprot.2008.217
Dec 18, 2008 · Furthermore, the more standard high-pressure gene gun (e.g., the Biolistic PDS-1000-He Particle Delivery System), with its fixed pressure system, produces extensive damage in Arabidopsis leaf ...
https://www.youtube.com/watch?v=gnz5zlzsRYw
Nov 03, 2015 · This video provides an overview of the Helios Gene Gun Biolistic Particle Transfection System, including instrument setup, microcarrier and cartridge preparation, and instrument operation.
https://www.researchgate.net/publication/11166861_Biolistic_transformation_of_Trichoderma_reesei_using_the_Bio-Rad_seven_barrels_Hepta_Adaptor_system
Effective biolistic transformation of intact conidia from the filamentous fungus Trichoderma reesei was achieved using the Bio-Rad Hepta Adaptor system with seven barrels for particle launch.
https://link.springer.com/protocol/10.1007/978-1-4939-7315-6_2
Disarmed Agrobacterium strains harboring standard binary vectors and the biolistic gun system Bio-Rad PDS-1000/He are used as gene delivery systems. The herbicide resistant bar gene and selection agent bialaphos are used for identifying putative transgenic type I callus events.
http://sites.clas.ufl.edu/mcdaniellab/files/Transformation-Biolistic.pdf
Assemble the microcarrier, disruption disk, and stopping grid into the particle delivery system according to the manufacturer’s instructions. 6. Place the protonemal tissue so that it is ~150 mm below the stopper plate of the PDS-1000/He biolistic particle delivery system. Make two discharges into each dish, moving the position of the
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