We have collected information about Anionic Liposome Delivery System For Dna Transfection for you. Follow the links to find out details on Anionic Liposome Delivery System For Dna Transfection.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2751225/
Anionic lipoplexes were formed by complexation between anionic liposomes and plasmid DNA molecules encoding green fluorescence protein (GFP) using Ca 2+ ions. Transfection and toxicity were evaluated in CHO-K1 cells using flow cytometry and propidium iodide staining, respectively.Author: Siddhesh D. Patil, David G. Rhodes, Diane J. Burgess
https://link.springer.com/article/10.1208/aapsj060429
Dec 01, 2004 · Efficient and relatively safe DNA transfection using anionic lipoplexes makes them an appealing alternative to be explored for gene delivery. The present study investigates the use of novel anionic lipoplexes composed of physiological components for plasmid DNA delivery …Author: Siddhesh D. Patil, David G. Rhodes, Diane J. Burgess
https://www.ncbi.nlm.nih.gov/pubmed/15760094
Oct 15, 2004 · Efficient delivery of plasmid DNA and subsequent GFP expression was achieved using anionic lipoplexes. Transfection efficiency increased with Ca2+ concentration up to 14 mM Ca2+, where transfection efficiency was 7-fold higher than in untreated cells, with minimum toxicity.Author: Siddhesh D. Patil, David G. Rhodes, Diane J. Burgess
https://www.researchgate.net/publication/7974549_Anionic_liposomal_delivery_system_for_DNA_transfection
Anionic lipoplexes were formed by complexation between anionic liposomes and plasmid DNA molecules encoding green fluorescence protein (GFP) using Ca2+ ions. Transfection …
https://freepaper.me/downloads/abstract/10.1208/aapsj060429
Anionic liposomal delivery system for DNA transfection The present study investigates the use of novel anionic lipoplexes composed of physiological components for plasmid DNA delivery into …
http://europepmc.org/abstract/MED/15760094
Jan 01, 2004 · Efficient delivery of plasmid DNA and subsequent GFP expression was achieved using anionic lipoplexes. Transfection efficiency increased with Ca2+ concentration up to 14 mM Ca2+ , where transfection efficiency was 7-fold higher than in untreated cells , with minimum toxicity.
https://www.hindawi.com/journals/jdd/2011/326497/
Several divalent cations have been tested for use in anionic lipoplexes such as Ca 2+ , Mg 2+ , Mn 2+ , and Ba 2+ , but it has been observed that the use of Ca 2+ yielded the highest transfection efficiency due to its higher DNA binding affinity [70, 71].Author: Daniel A. Balazs, Wt. Godbey
http://www.cell-transfection.com/home-2/cationic-lipid-liposome-transfection/
Liposomes generate a low toxicity response and are used in live subjects as a delivery vehicle. Considerations. Cationic lipids inhibit entry of foreign DNA into the nucleus, and therefore the rate of stable transfection remains relatively low. Advanced techniques are often required for successful generation of stable cell lines.
https://www.researchgate.net/publication/51049116_Liposomes_for_Use_in_Gene_Delivery
Liposomes are often used to deliver a molecular cargo such as DNA for therapeutic benefit. The lipids used to form such lipoplexes can be cationic, anionic, neutral, or a mixture thereof. Herein physical packing parameters and specific lipids used for gene delivery will be discussed, with lipids classified according to overall charge.
https://www.sciencedirect.com/science/article/pii/S181808761300010X
Cationic liposomes have been studied as a means to enhance the stability of nucleic acid therapeutics in the bloodstream and improve their cellular delivery. As nucleic acid therapeutics, siRNA and plasmid DNA have been extensively tested for delivery using cationic liposomes.Author: Gayong Shim, Mi-Gyeong Kim, Joo Yeon Park, Yu-Kyoung Oh
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