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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679364/
In addition, viral vectors encoding shRNAs have shown promise for in vivo delivery; however, most of these studies have utilized adenoviral delivery of shRNA, which has well-known toxic effects in the animal . Recently, adeno-associated viral vectors (AAV) have been designed with less toxicity and adequate shRNA delivery .Author: Chris B. Moore, Elizabeth H. Guthrie, Max Tze-Han Huang, Debra J. Taxman
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862356/
Aug 15, 2006 · In summary, the results of the present study indicate that: 1) lentiviral-mediated gene delivery is best suited for situations when long-term delivery of a transgene is needed, whereas ex vivo retroviral delivery system is more appropriate for transient delivery of therapeutic molecules, with adenoviral-driven expression intermediate; 2) the ...Author: Ahmed A. Abdellatif, Jennifer L. Pelt, Richard L. Benton, Russell M. Howard, Pantelis Tsoulfas, Peip...
https://www.thermofisher.com/order/catalog/product/K494100
The pAd/BLOCK-iT™-DEST RNAi Gateway® Vector can be used for the efficient delivery and transient expression of a short hairpin RNA (shRNA) in vivo from an adenoviral vector.A novel cloning process places an ~50-bp DNA oligonucleotide immediately following a …
https://www.thermofisher.com/us/en/home/life-science/rnai/virus-based-rnai-analysis/lentiviral-shrna-and-mirna-analysis.html
13 rows · Viral delivery can be the best option in virtually any mammalian cell type, including hard-to …
https://www.takarabio.com/products/viral-transduction/adenoviral-vector-systems/adeno-xt-adenoviral-system-3-(tet-on%C2%AE-3g-inducible)
Adeno-X Adenoviral System 3 (CMV) provides the components necessary to generate a constitutive expression system in an adenoviral vector format. pAdenoX-CMV (included in the vector set) is a prelinearized, adenoviral vector that is ready for the insertion of your gene of interest via our In-Fusion HD PCR Cloning technology.
https://www.vectorbiolabs.com/adenovirus-amplification-service/
Large Scale Adenovirus Amplification & CsCl Purification. This service is ideal for those who require recombinant adenovirus for in vivo studies or just require high-titer purified viruses.. First, we infect ~4000cm 2 of 293 cell monolayer through a series of amplifications from customer-provided viral stock or any adenovirus from our catalog. Then, once cytopathic effects begin to manifest ...
https://www.sciencedirect.com/science/article/pii/S0304383515003821
We also demonstrated that delivery of MMP14-targeting shRNA by a fiber-modified adenoviral vector to the glioma cells effectively suppresses their proliferation in vitro and in vivo. Thus our data indicate that inhibition of MMP14 expression in tumors in combination with glioma virotherapy could be effectively utilized to suppress angiogenesis ...Author: Ilya Ulasov, Anton V. Borovjagin, Natalya Kaverina, Brett Schroeder, Nameeta Shah, Biaoyang Lin, Ana...
https://www.vectorbiolabs.com/adenovirus-construction-service/
Adenovirus construction is a tedious and time-consuming process. Here at Vector Biolabs, we have developed the Ad-HQ system: a fail-proof construction method that guarantees adenovirus generation. Since 2004, we have successfully constructed tens of thousands of viruses for gene over-expression, shRNA silencing, and gRNA gene editing.
https://www.researchgate.net/post/Can_anyone_explain_the_incompatible_results_obtained_when_using_Adenoviral_delivered_siRNA_vs_siRNA_transfection_with_lipofectamine
The target sequence of your shRNA for adenoviral delivery and your siRNA will be different. There is a possibility that you observed off-target effects in one of your approaches.
https://www.researchgate.net/profile/Miroslava_Ogorelkova/publication/7405815_DNA_delivery_to_cells_in_culture_generation_of_adenoviral_libraries_for_high-throughput_functional_screening/links/0046351eeba2d2a656000000.pdf
Traditionally, viral delivery of antisense RNA and, more recently, small interfering RNA (siRNA) molecules in the form of small hairpin RNAs (shRNA) has been used as a strategy to achieve gene ...Author: Miroslava Ogorelkova, John Zwaagstra, Seyyed Mehdy Elahi, Christel Dias, Claire Guilbaut, Rita Lo, C...
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