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https://www.cdc.gov/vaccinesafety/pdf/Vaccine-Safety-Process-508.pdf
the vaccine life cycle safety at every phase guide acip advisory committee on immunization practices bla biologics license application . cdc . centers for disease . cdc + fda . control and prevention . fda . food and drug administration . ind . investigational new drug application . adverse events . vaccine. development.
https://en.wikipedia.org/wiki/Vaccinia
Vaccinia virus (VACV or VV) is a large, complex, enveloped virus belonging to the poxvirus family. It has a linear, double-stranded DNA genome approximately 190 kbp in length, which encodes approximately 250 genes.The dimensions of the virion are roughly 360 × 270 × 250 nm, with a mass of approximately 5–10 fg. The vaccinia virus is the source of the modern smallpox vaccine, which the ...
https://pubmed.ncbi.nlm.nih.gov/15280497/
The 70-amino-acid A13L protein is a component of the vaccinia virus membrane. We demonstrate here that the protein is expressed at late times of infection, undergoes phosphorylation at a serine residue (s), and becomes encapsidated in a monomeric form. Phosphorylation is dependent on Ser40, which lies within the proline-rich motif SPPP.
https://pubmed.ncbi.nlm.nih.gov/26636113/
Vaccinia virus can replicate the genome in the presence of Rep78. (a) HeLa cells were transfected with pCI-Rep/Cap, pictured, and pscAAV-CB-EGFP. Upon transfection, cells were infected with adenovirus (Ad) or vaccinia virus (VV) or mock infection (mock) and were harvested at 48 hours post-infection.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55651/
Vaccinia virus (vv), a member of the poxvirus family, is unique among most DNA viruses in that its replication occurs in the cytoplasm of the infected host cell. ... The vv cytoplasmic life cycle is initiated upon virus entry at the plasma membrane in a poorly understood process (Krijnse Locker et al., 2000), but which results in the delivery ...
https://jitc.biomedcentral.com/articles/10.1186/s40425-018-0495-7
Vaccinia virus life cycle. A diagram of the infected cell with an exaggerated view of the cellular compartments, including the ER (endoplasmic reticulum), CGN (cis-Golgi network), C (cis-Golgi), M (medial-Golgi), T (trans-Golgi) and TGN (trans-Golgi network), is shown. Also shown are the major stages of the viral life cycle.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2654849/
Oncolytic viral therapy exploits the life cycle of viruses to specifically infect, replicate within, and lyse cancer cells. Many virus types, including adenoviruses ( 1 ), herpes simplex viruses ( 2 ), Newcastle disease virus ( 3 ), myxoma virus ( 4 ), vaccinia virus (VACV) ( 5 ), and vesicular stomatitis virus ( 6 ), are being investigated as ...
http://dnareplication.cshl.edu/content/free/chapters/27_traktman.pdf
THE POXVIRAL LIFE CYCLE Poxviruses, of which vaccinia is the prototype, are complex DNA viruses that display an unusual degree of physical and genetic autonomy from the host cell. The vaccinia genome encodes most, if not all, of the func- tions required for three temporally regulated phases of …
https://www.science.org/doi/10.1126/science.1122411
Indeed F11L, which is expressed from as early as 2 hours after infection, is associated with perinuclear viral factories (fig. S5). To elucidate the function of F11L in the vaccinia life cycle, we set out to delete the F11L gene in the WR genome by homologous recombination. However, in agreement with Kato et al.
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