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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2692493/
Jun 01, 2009 · Direct local injection of siRNA, with or without a delivery system, into the tumor has been an effective anticancer approach in vivo. Delivery of siRNA targeting RasA and RasC in a xenograft breast cancer model in mice has been shown to successfully reduced tumor volume 74. However, clinically, only a few tumors are suitable for local delivery; systemic approaches described above are …Author: Kun Gao, Leaf Huang
https://www.thermofisher.com/us/en/home/life-science/rnai/delivering-rnai-to-cells-transfection-and-viral-delivery.html.html
Viral delivery can also be used to create stable cell lines with inducible RNAi expression or to express RNAi sequences with tissue-specific promoters. Importance of minimizing transfection-mediated cytotoxicity The delivery of RNAi reagents, or the delivery method itself, can give rise to cytotoxicity in gene silencing experiments.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1559924/
Jun 25, 2006 · Delivery of chemically synthesized siRNA In this approach, reconstituted viral envelopes derived from influenza virus are used to encapsulate and deliver siRNAs. The reconstituted membrane vesicles contain the influenza virus spike protein hemagglutinin and additionally added cationic lipids.Author: Sabrina Oliveira, Gert Storm, Raymond M. Schiffelers
https://www.alliedacademies.org/articles/rethinking-delivery-viral-vectors-and-rna-interference-7646.html
Viral vectors are a promising alternative to deliver short hairpin RNA (shRNA) expression cassettes. Many corporate researchers are reluctant to consider viral vectors as a means of delivery due to the poor reputation of gene therapy, as a consequence of adverse events that occurred in early studies.Author: Jens Kurreck
https://www.intechopen.com/books/rna-interference/non-viral-sirna-and-shrna-delivery-systems-in-cancer-therapy
Development of appropriate delivery systems is an important issue in the use of siRNA-based therapeutics. Non-viral delivery systems have great potential for safe and effective delivery of siRNA therapeutics to tumor cells. Nanocarriers such as nanoplexes, lipoplexes, nanoparticles, and liposomes have been commonly used for siRNA delivery.Author: Emine Şalva, Ceyda Ekentok, Suna Özbaş Turan, Jülide Akbuğa
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