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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · Nonviral systems comprise all the physical and chemical systems except viral systems and generally include either chemical methods, such as cationic liposomes and polymers, or physical methods, such as gene gun, electroporation, particle bombardment, ultrasound utilization, and …
https://seekingalpha.com/article/4305556-gene-therapy-series-2-delivery-systems-in-gene-therapy-including-viral-vectors
Nov 11, 2019 · Examples of gene therapy methods using ex vivo delivery include CAR-T therapies like Yescarta and Kymriah. In vivo delivery: By definition, this means inside the body. In this method…
https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/transfection-basics/gene-delivery-technologies.html
Highest efficiency amongst gene delivery methods (80–90% transduction efficiency in primary cells) Works well with difficult to transfect cell types. Can be used for in vivo delivery of nucleic acids. Can be used for making stable cell lines (retroviral vectors) or for transient expresssion (adenoviral vectors)
https://www.ncbi.nlm.nih.gov/pubmed/23437936
Non-viral gene delivery methods. Wang W(1), Li W, Ma N, Steinhoff G. Author information: (1)Reference- and Translation Center for Cardiac Stem Cell Therapy, Department of Cardiac Surgery, University of Rostock, 18057 Rostock, Germany.Author: Weiwei Wang, Wenzhong Li, Nan Ma, Gustav Steinhoff
https://www.tandfonline.com/doi/figure/10.1080/14712598.2018.1416089?scroll=top&needAccess=true
Introduction: Adeno-associated viral (AAV) vector-mediated gene delivery to the spinal cord has finally entered the pathway towards regulatory approval. Phase 1 clinical trials using AAV gene thera...
https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/transfection-basics/gene-delivery-technologies/viral-delivery.html
For cell types not amenable to lipid-mediated transfection, viral vectors are often employed. Virus-mediated transfection, also known as transduction, offers a means to reach hard-to-transfect cell types for protein overexpression or knockdown, and it is the most commonly used method in clinical research (Glover et al., 2005; Pfeifer and Verma, 2001).
https://www.intechopen.com/books/gene-therapy-tools-and-potential-applications/non-viral-delivery-systems-in-gene-therapy
Non-viral methods for transfection 3.1. Inorganic particles. Inorganic nanoparticles are nanostructures varying in size, shape and porosity,... 3.2. Synthetic or natural biodegradable particles. 3.3. Physical methods for gene delivery. Gene delivery using physical principles has attracted ...Author: Alicia Rodríguez Gascón, Ana del Pozo-Rodríguez, María Ángeles Solinís
https://www.slideshare.net/Aparajitha_Anne/gene-delivery-system
May 19, 2012 · Inorganic Nanoparticles:It one of the non-viral gene delivery system where we use gold .silicaand iron oxide and calcium phosphate some of the benefits are storagestability, low manufacturing cost and often time, low immunogenicity.
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