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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2923131/
Results. A retrotransposon vector system was developed for gene delivery in plants based on the Tnt1 element from Nicotiana tabacum.Mini-Tnt1 transfer vectors were constructed that lack coding sequences yet retain the 5' and 3' long terminal repeats (LTRs) and adjacent cis sequences required for reverse transcription.The internal coding region of Tnt1 was replaced with a neomycin ...Author: Yi Hou, Yi Hou, Jyothi Rajagopal, Phillip A Irwin, Daniel F Voytas
https://www.hindawi.com/journals/bmri/2015/932161/
As a result, an Agrobacterium-based gene delivery via agroinfiltration has become the favorable gene delivery method for transient expression in plants [7, 8]. 3. Agroinfiltration for Expression of Recombinant Proteins. On a per cell basis, the yield of a RP is usually higher in transient expression than that in transgenic plants .Author: Qiang Chen, Huafang Lai
http://www.eagri.org/eagri50/GPBR311/lec23.pdf
Gene delivery methods in plants For production of transgenic animals, DNA is usually microinjected into pronuclei of embryonic cells at a very early stage after fertilization, or alternatively gene targeting of embryo stem (ES) cells is employed. This is possible in animals due to the
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449920/
May 17, 2015 · Recombinant proteins are primarily produced from cultures of mammalian, insect, and bacteria cells. In recent years, the development of deconstructed virus-based vectors has allowed plants to become a viable platform for recombinant protein production, with advantages in versatility, speed, cost, scalability, and safety over the current production paradigms.Author: Qiang Chen, Huafang Lai
https://mobilednajournal.biomedcentral.com/articles/10.1186/1759-8753-1-19
Aug 02, 2010 · A retrotransposon vector system was developed for gene delivery in plants based on the Tnt1 element from Nicotiana tabacum. Mini-Tnt1 transfer vectors were constructed that lack coding sequences yet retain the 5' and 3' long terminal repeats (LTRs) and adjacent cis sequences required for reverse transcription.Author: Yi Hou, Yi Hou, Jyothi Rajagopal, Phillip A Irwin, Daniel F Voytas
https://www.researchgate.net/publication/45460374_Retrotransposon_vectors_for_gene_delivery_in_plants
A retrotransposon vector system was developed for gene delivery in plants based on the Tnt1 element from Nicotiana tabacum. Mini-Tnt1 transfer vectors were constructed that lack coding sequences ...
http://www.biologydiscussion.com/genetics/engineering/methods-of-gene-transfer-in-plants-2-methods/10824
Methods of Gene Transfer in Plants: 2 Methods. Article Shared by. ... Gemini viruses and RNA viruses that are used as vectors for gene transfer in plants are briefly described. ... This technique can be used for the delivery of DNA into intact plant cells and protoplasts.
https://www.researchgate.net/profile/Daniel_Voytas/publication/45460374_Retrotransposon_vectors_for_gene_delivery_in_plants/links/0f3175346a2d881684000000.pdf?disableCoverPage=true
RESEARCH Open Access Retrotransposon vectors for gene delivery in plants Yi Hou1,2, Jyothi Rajagopal1,3, Phillip A Irwin1,4, Daniel F Voytas1* Abstract Background: Retrotransposons are abundant ...
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