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https://www.ephlebotomytraining.com/phlebotomy-tubes-explained/
EDTA tubes are used in direct sampling analyzers without the need to open them. For up to 24 hours, erythrocytes, white blood cells and platelets remain stable in blood anticoagulated with EDTA, but blood smears must be prepared 3 hours after specimen is collected.
https://www.catholicmedicalcenter.org/CatholicMedicalCenter/media/CMCE-Media-Library/CareTreatment/Laboratory/info%20for%20HCP/speccoll_rev.pdf
Gold and red top tubes should be allowed to clot for a minimum of 30 minutes and centrifuged within a maximum of 2 hours after collection. Serum for the red top tube must be removed as
https://www.labcorp.com/resource/blood-specimens-chemistry-and-hematology
Red-top tube: Contains no anticoagulant or preservative. Use: Serum or clotted whole blood. Serum must be separated from cells within 45 minutes to two hours depending on the test(s).
http://www.pathology.uci.edu/services/specimen-containers.asp
If the specimen requirement for a test is red-top tube(s), do not use gold-top/SST tube(s). NOTE: Invert the tube to activate the clotting; let stand for 20-30 minutes before centrifuging for 10 minutes. If frozen serum is required, pour off serum into plastic vial and freeze. Do not freeze Vacutainer® tubes. Red-top tube, plastic
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http://a1.mayomedicallaboratories.com/webjc/attachments/117/1548f87-specimen-collection-and-preparation.pdf
Hematology Testing (Lavender-Top Tubes Containing EDTA for Hemoglobin/Hematocrit, CBC, etc.): Specimens may be assayed up to 36 hours after draw, if refrigerated. Tubes must be well mixed immediately after draw. The 4-mL lavender-top (EDTA) tubes must be at least half full with 2 mL of whole blood; the 2-mL pediatric lavender-top
https://www.expresslabidaho.com/wp-content/uploads/2019/07/Instructions-for-Centrifuging-Lab-Specimens.pdf
move freely by inversion (do not shake the tube) before spinning. SST/Red tops should be spun for 12-15 minutes. Light green top tubes may be spun immediately. Spin for 5 minutes at a speed of 3600 rpm. Delayed Pickup Specimens that will not be received by the lab within 3 hours need to be spun down and refrigerated
https://www.vet.cornell.edu/sites/default/files/blood_tubes_and_labeling_guidelines.pdf
*Serum separator tubes (tiger top) can be substituted for red top tubes in some instances but should be avoided for certain endocrinology and clinical pathology tests. Please centrifuge the serum separator tubes after a clot forms,transfer the supernatant to another tube and label the new tubewith owner, animal ID, and as SERUM.
https://www.acllaboratories.com/static/documents/Dos-Donts-Specimen-Handling-Flyer.pdf
DO allow red top tubes to clot in an upright position at room temperature for 60 minutes, gold top for 30 minutes. Centrifuge for the preprogrammed time or 10 minutes for red or gold top tubes, 10 minutes for green, and 15 minutes for BD Blue top tubes. If minimum g-force (1300g) cannot be achieved, spin for 20 minutes. Do not use a manual
https://www.acllaboratories.com/static/documents/2072_BD-Tube-Conversion-Chart_FINAL-10.2014.pdf
Tube Conversion Guide NOTE: Gold Gel tubes should clot for 30 minutes before centrifugation. Red, no additive tubes should clot for 60 minutes before centrifugation. Tubes should be centrifuged within 2 hours of collection. Gel tubes should not be spun in a refrigerated centrifuge.
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