Transit-Qr Hydrodynamic Delivery Solution

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TransIT-QR Delivery Solution - Mirus Bio

    https://www.mirusbio.com/products/in-vivo/transit-qr-delivery-solution
    TransIT®-QR Delivery Solution Effectively Delivers the Label IT® Cy®3 RNAi Delivery Control to Hepatocytes. TransIT®-QR Delivery Solution was used to deliver 25 µg of Label IT® Cy®3 RNAi Delivery Control (red) to a mouse using hydrodynamic delivery via the tail vein. Forty-five minutes post-injection the liver was harvested and sections were fixed then counterstained to stain the nuclei ...

www.mirusbio.com

    https://www.mirusbio.com/assets/protocols/ml041_transit_qr_delivery_solution.pdf
    Title: Protocol Author: Mirus Bio LLC (https://www.mirusbio.com) Subject: Protocol Created Date: 4/7/2020 9:00:06 PM

Mirus Bio TransIT-QR Hydrodynamic Delivery Solution and ...

    https://www.fishersci.com/shop/products/mirus-bio-i-trans-i-it-qr-hydrodynamic-delivery-solution-starter-kit-2/p-3082119
    Shop online for a wide selection of Mirus Bio TransIT-QR Hydrodynamic Delivery Solution and Starter Kit A nontoxic, “Quick Recovery” in vivo delivery solution

TransIT-QR Hydrodynamic Delivery Solution|タカラバイオ株式会社

    http://catalog.takara-bio.co.jp/product/basic_info.php?unitid=U100004896
    TransIT-QR(Quick Recovery) Hydrodynamic Delivery Solutionは、核酸と直接混合し、マウスの尾静脈に注射することによりマウス生体細胞に核酸を導入する試薬である。本試薬は注射後のマウスが迅速に回復するという特長をもっており、安全に核酸の導入が行える。siRNAおよびDNAの導入に使用すること …

TransIT®-QR Delivery Solution and Starter Kit, Mirus Bio VWR

    https://us.vwr.com/store/product/18905872/transit-qr-delivery-solution-and-starter-kit-mirus-bio
    A nontoxic, Quick Recovery in vivo delivery solution that delivers DNA or siRNA to the livers of laboratory mice using hydrodynamic tail vein injection.VWR offers transfection reagents for numerous applications, including bioproduction, in vivo work, viral production, DNA and siRNA transfection, CRISPR/Cas 9 and more. While considering a suitable transfection reagent, it is important to ...Brand: Transit®

Mirus Bio TransIT-EE Hydrodynamic Delivery Solution and ...

    https://www.fishersci.com/shop/products/mirus-bio-i-trans-i-it-ee-hydrodynamic-delivery-solution-starter-kit-2/p-3082126
    Naked Nucleic Acid Delivery: Achieve increased levels of gene expression than compared to TransIT-QR Delivery Solution, polylysine and PEI methods; Versatile Platform: Suitable for DNA delivery using methods including hydrodynamic tail vein injection to the mouse strain of your choice

Establishment of a tTA-dependent photoactivatable Cre ...

    https://www.sciencedirect.com/science/article/pii/S0006291X2030485X
    The TRE-PA-Cre:Rosa26-tdTomato mice were rested for at least 24 h in a cage. Ten μg of tTA expression vector (Addgene#16541) was intravenously injected using a TransIT-QR Hydrodynamic Delivery Solution (Mirus Bio LLC, Madison, WI, USA) according to the manufacturer’s protocol and the hydrodynamic procedure .Author: Tomoka Takao, Yuichi Hiraoka, Kenji Kawabe, Daisuke Yamada, Lu Ming, Kohichi Tanaka, Moritoshi Sato,...

A non-inheritable maternal Cas9 ... - PubMed Central (PMC)

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4730228/
    Hydrodynamic gRNAs delivery into Cas9/+ mice and indel mutational analysis in hepatocytes. The procedure for hydrodynamic gRNA delivery is shown in Fig. 6a. Hydrodynamic gRNA delivery was carried out using the TransIT-QR Hydrodynamic Delivery Solution kit (Mirus Bio LLC; Madison, WI, USA) according to the manufacturer’s protocol.Author: Takayuki Sakurai, Akiko Kamiyoshi, Hisaka Kawate, Chie Mori, Satoshi Watanabe, Megumu Tanaka, Ryuich...

Ischemia/Reperfusion-Inducible Protein Modulates the ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3773277/
    Jul 01, 2013 · The pLiver-mIrip plasmids (90 μg/ml) were dissolved in TransIT-QR Hydrodynamic Delivery Solution (Mirus Bio LLC., Pittsburgh, PA). Total volume needed per mouse (in ml) was calculated as mouse weight (g)/10 plus 0.1 ml of delivery solution which was to compensate the volume remaining in the syringe and needle after injection.Author: Qing Li, Hyekyung Yang, Xiujuan Peng, Dong Guo, Zhongqi Dong, James E. Polli, Yan Shu

m-Calpain antagonizes RhoA overactivation and endothelial ...

    https://academic.oup.com/cardiovascres/article/85/3/530/279089
    Sep 13, 2009 · For in vivo experiments, mice were briefly anaesthetized with diethyl ether, followed by transfection with a small interfering RNA (siRNA) against m-calpain (3 µg/body) or non-silencing control RNA (3 µg/body) involving TransIT-QR Hydrodynamic delivery solution (Mirus Bio) in accordance with the manufacturer's instructions. The mice were ...Author: Takuro Miyazaki, Kazuo Honda, Hisayuki Ohata

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