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https://www.uwyo.edu/molb2210_lab/info/biochemical_tests.htm
Thus, after the first few hours of incubation, the tube will be entirely yellow. At this point, when the glucose has been all used up, the organism must choose another food source. If the organism can ferment lactose, this is the sugar it will choose. Lactose fermentation will continue to produce acidic byproducts and the media will remain ...
http://web.mta.info/nyct/bus/schedule/staten/sim003cur.pdf
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https://quizlet.com/280987846/lab-quiz-3-flash-cards/
The sample was incubated less than 24 hours, which allowed for only limited fermentation. The test organism ferments sucrose best under reduced oxygen conditions. The organism is limited in its ability to ferment sucrose. Since diffusion is restricted within the Durham tube and it contains only a small volume of media, it turned yellow first.
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