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https://www.sciencedirect.com/science/article/pii/S0091679X04770193
Publisher Summary This chapter focuses on the sleeping beauty transposon for efficient gene delivery. The core use of the Sleeping Beauty (SB) transposon in zebrafish has …Author: Spencer Hermanson, Ann E. Davidson, Sridhar Sivasubbu, Darius Balciunas, Stephen C. Ekker
https://www.researchgate.net/publication/8126873_Sleeping_Beauty_Transposon_for_Efficient_Gene_Delivery
In principle this gene-breaking strategy is scalable for functional genomics screens and can be used in Sleeping Beauty transposon and other gene delivery systems in the zebrafish.
https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(18)30019-4
Apr 04, 2018 · The Sleeping Beauty (SB) transposon system is a non-viral gene delivery platform that combines simplicity, inexpensive manufacture, and favorable safety features in the context of human applications. However, efficient correction of hematopoietic stem and progenitor cells (HSPCs) with non-viral vector systems, including SB, demands further refinement of gene delivery techniques.Author: Marta Holstein, Cristina Mesa-Nuñez, Csaba Miskey, Elena Almarza, Valentina Poletti, Marco Schmeer, ...
https://www.sciencedirect.com/science/article/pii/S1525001603002168
To produce Sleeping Beauty -based HITT-vectors, we inserted the transposase coding sequence flanked by a short promoter insertion linker (PIL) 227 bp upstream of the left inverted repeat of a transposon (T/hAAT) containing the hAAT gene expressed from an RSV promoter. The two genes were oriented in the same direction.Author: Jacob Giehm Mikkelsen, Stephen R Yant, Leonard Meuse, Zan Huang, Hui Xu, Mark A Kay
https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(03)00216-8
Helper-Independent sleeping beauty Transposon–Transposase vectors for efficient nonviral gene delivery and persistent gene expression in vivo Previous Article Transfection efficiency and toxicity following delivery of naked plasmid DNA and cationic lipid–DNA complexes to ovine lung segmentsAuthor: Jacob Giehm Mikkelsen, Stephen R Yant, Leonard Meuse, Zan Huang, Hui Xu, Mark A Kay
https://www.ncbi.nlm.nih.gov/pubmed/14529839
Helper-Independent Sleeping Beauty transposon-transposase vectors for efficient nonviral gene delivery and persistent gene expression in vivo. Mikkelsen JG(1), Yant SR, Meuse L, …Author: Jacob Giehm Mikkelsen, Stephen R Yant, Leonard Meuse, Zan Huang, Hui Xu, Mark A Kay
https://www.sciencedirect.com/science/article/pii/S1525001603003770
This element, the Sleeping Beauty transposon, shows efficient transposition in cells of a wide range of vertebrates, including humans. Sleeping Beauty transposition is a cut-and-paste process, during which the element “jumps” from one DNA molecule to another.Author: Zsuzsanna Izsvák, Zsuzsanna Izsvák, Zoltán Ivics
https://www.sciencedirect.com/science/article/pii/S0012160603004391
Nov 15, 2003 · These results indicate that Sleeping Beauty is an efficient tool for gene delivery and expression and provides new opportunities for large-scale functional genomics in zebrafish.Author: Ann E Davidson, Darius Balciunas, Deanna Mohn, Jennifer Shaffer, Spencer Hermanson, Sridhar Sivasubb...
https://mayoclinic.pure.elsevier.com/en/publications/sleeping-beauty-transposon-for-efficient-gene-delivery
TY - CHAP. T1 - Sleeping Beauty Transposon for Efficient Gene Delivery. AU - Hermanson, Spencer. AU - Davidson, Ann E. AU - Sivasubbu, Sridhar. AU - Balciunas, DariusAuthor: Spencer Hermanson, Ann E. Davidson, Sridhar Sivasubbu, Darius Balciunas, Stephen C. Ekker
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