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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1559924/
Jun 25, 2006 · To overcome these barriers several strategies have been developed, such as chemical modifications of siRNA, viral nucleic acid delivery systems, and nonviral nucleic acid delivery systems. Here, we discuss progress that has been made to improve targeted delivery of …Author: Sabrina Oliveira, Gert Storm, Raymond M. Schiffelers
https://www.thermofisher.com/us/en/home/life-science/rnai/delivering-rnai-to-cells-transfection-and-viral-delivery.html.html
Viral delivery can also be used to create stable cell lines with inducible RNAi expression or to express RNAi sequences with tissue-specific promoters. Importance of minimizing transfection-mediated cytotoxicity. The delivery of RNAi reagents, or the delivery method itself, can give rise to cytotoxicity in gene silencing experiments.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2977003/
Jun 11, 2010 · The site of action of siRNA therapeutics is the cytosol. The barriers to siRNA delivery are multiple and depend on the targeted organs and the administration routes. In general, locoregional delivery of siRNA has fewer barriers compared to systemic delivery.Author: Jie Wang, Ze Lu, M. Guillaume Wientjes, Jessie L.-S. Au
https://www.alliedacademies.org/articles/rethinking-delivery-viral-vectors-and-rna-interference-7646.html
These shRNAs are processed by the cellular machinery into siRNAs, which then knockdown the target gene. This strategy not only allows prolonged silencing, as the shRNAs are continuously produced, it also opens up the possibility of using viral vectors for the delivery of double-stranded RNA molecules.Author: Jens Kurreck
https://www.thermofisher.com/us/en/home/references/ambion-tech-support/rnai-sirna/tech-notes/enhanced-sirna-delivery-and-long-term-gene-silencing.html
(In general, chemically synthesized siRNAs are a better choice for transient experiments in most immortalized cell lines.) In contrast, viral approaches facilitate effective siRNA delivery into many difficult-to-transfect cell types, including primary cells, because infection is a much more efficient delivery mechanism than transfection.
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