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https://local.yahoo.com/info-10118829-pro-seq-incorporated-boxford
Management & Consulting, Market Research; Address: 3 King George Dr, Boxford, MA 01921; Cross Streets: Between Killam Hill Rd and King William Ct; Phone: (978) 213-9911
https://www.illumina.com/science/sequencing-method-explorer/kits-and-arrays/pro-seq.html
Precision nuclear run-on sequencing (PRO-Seq) maps RNAP active sites with base-pair resolution. This approach is similar to GRO-Seq, but it provides the added benefit of single-base resolution. RNAPII initiation sites can be mapped using a modified protocol named PRO-cap. In PRO-seq, a run-on reaction is carried out with biotin-NTPs (either all ...
https://hpc.nih.gov/apps/proseq.html
proseq-2.0 is a pipeline for preprocesses and alignment of run-on sequencing (PRO/GRO/ChRO-seq) data from Single-Read or Paired-End Illumina Sequencing Useful references: (GRO-seq:) Leighton J. Core, Joshua J. Waterfall, John T. Lis Nascent RNA Sequencing Reveals Widespread Pausing and Divergent Initiation at Human Promoters
http://enseqlopedia.com/wiki-entry/rna-sequencing-methods/rna-transcription/pro-seq/
PRO-Seq. Precision nuclear run-on sequencing (PRO-Seq) maps RNAPII pause sites with base-pair resolution during RNA transcription (Kwak et al., 2013) (Mahat et al., 2016). This approach is similar to GRO-Seq, but it provides the added benefit of single-base resolution. RNAPII initiation sites can be mapped using a modified protocol named PRO-cap.
https://ntc.hms.harvard.edu/pro-seq
Method Description PRO-seq (Precision Run-On Sequencing) offers single nucleotide resolution of nascent RNA 3' ends. This information can be used to identify candidate enhancer RNAs and profile the immediate transcriptional effects of experimental perturbations such as drug-treatment, cell differentiation, or protein degradation.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5502525/
Seed cells at a concentration that will enable them to reach ~80% confluency in 24 hours. For a PRO-seq experiment using adherent fibroblasts, 4–6 150mm cell culture dishes yield sufficient cells (~10 7 cells, see Experimental Design for further details). For yeast cells, ...
https://www.nature.com/articles/nprot.2016.086/
Mahat et al. describe how to map the genome-wide positions of active RNA polymerases using a modified nuclear run-on approach called PRO-seq. Details for PRO-cap, a …
http://peppro.databio.org/
PEPPRO is a pipeline for nascent RNA sequencing data. It can process PRO-seq, GRO-seq, and ChRO-seq data and is optimized on unique features of nascent RNA to be fast and accurate. It performs variable-length UMI adapter removal, read deduplication, trimming, mapping, QC, and signal tracks (bigWig) for plus and minus strands using mappability-scaled or unscaled read counts.
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https://www.bioinfor.com/
Advanced Solutions. Advanced LC-MS/MS software for data analysis. Sensitive and accurate protein identification and quantification. Comprehensive antibody characterisation. Complete and vendor neutral solution for DDA & DIA proteomics. Continuous research, advanced algorithms and state-of-the-art technology. Dedicated customer support.
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