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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151968/
Oct 16, 2007 · Protein delivery procedure. Cells were plated in order to reach approximately 70–80% confluency the day of protein delivery experiment. For one well of a 24-well plate, 0.5–8 μg of purified protein was diluted in 100 μL of Hepes buffer (20 mM, pH 7.4) in a 1.5 mL microcentrifuge tube, under sterile conditions.Author: Claire O. Weill, Stéphanie Biri, Abdennaji Adib, Patrick Erbacher
https://www.cell.com/cell/fulltext/S0092-8674(15)00315-3
Apr 23, 2015 · Here, we describe a method for the efficient delivery of native proteins and other macromolecules, such as small RNAs, into primary cells. We discovered that a combination of small molecules drives the highly efficient intracellular delivery of native proteins, independent of any transduction peptide.Author: Diego S. D’Astolfo, Romina J. Pagliero, Anita Pras, Wouter R. Karthaus, Hans Clevers, Vikram Prasad,...
http://www.genlantis.com/protein-delivery.html
Protein Delivery / Transfection Genlantis has developed a different kind of protein delivery approach using a novel reagent called BioPORTER® protein transfection reagent.BioPORTER reagent is a unique cationic lipid mixture that is hydrated with a solution of the protein that is to be delivered.
https://www.ncbi.nlm.nih.gov/pubmed/30398334
Dec 12, 2018 · Here, the genetic fusion of a supercharged polypeptide, called SCP, to a protein provides a generic method for intracellular protein delivery. It allows efficient protein endocytosis and endosomal escape and is capable of potently delivering various proteins with a range of charges, sizes, and bioactivities into the nucleus of living cells.Author: Jun Yin, Qun Wang, Shan Hou, Lichen Bao, Wenbing Yao, Xiangdong Gao
https://science.sciencemag.org/content/284/5418/1322.full
May 21, 1999 · Several Gram-negative pathogenic bacteria have evolved a complex protein secretion system termed type III to deliver bacterial effector proteins into host cells that then modulate host cellular functions. These bacterial devices are present in both plant and animal pathogenic bacteria and are evolutionarily related to the flagellar apparatus.Author: Jorge E. Galán, Alan Collmer
https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/protein-delivery
Devin M. Stranford, Joshua N. Leonard, in Advances in Genetics, 2017. 2.5 Protein. Direct protein delivery is an attractive method for bypassing the challenges associated with achieving transcription and translation in EV recipient cells. Recently, several methods have been developed to enrich specific proteins in EVs. One method specifically localizes proteins to exosomes by using a WW tag to ...
https://www.cell.com/trends/cell-biology/comments/S0962-8924(00)01771-2
Several proteins can traverse biological membranes through protein transduction. Small sections of these proteins (10–16 residues long) are responsible for this. Linking these domains covalently to compounds, peptides, antisense peptide nucleic acids or 40-nm iron beads, or as in-frame fusions with full-length proteins, lets them enter any cell type in a receptor- and transporter-independent ...Author: Steven R. Schwarze, Keith A. Hruska, Steven F. Dowdy
https://www.nature.com/articles/nmeth.2998
Jun 15, 2014 · A tetramethylrhodamine-labeled dimer of the cell-penetrating peptide TAT, named dfTAT, efficiently delivers proteins into live cells by facilitating endosomal escape. We report that a ...Author: Alfredo Erazo-Oliveras, Kristina Najjar, Laila Dayani, Ting-Yi Wang, Gregory A Johnson, Jean-Philipp...
https://www.pnas.org/content/113/11/2868
Mar 15, 2016 · In addition, modulation of protein charge through either genetic fusion of supercharged protein or complexation of Cas9 with its inherently anionic sgRNA allows highly efficient protein delivery and effective genome editing in mammalian cells and functional recombinase delivery in the rodent brain.Author: Ming Wang, John Zuris, Fantao Meng, Holly Anne Rees, Shuo Sun, Pu Deng, Yong Han, Xue Gao, Dimitra P...
https://pubs.rsc.org/en/content/articlelanding/2018/sc/c8sc00367j
Natural biological cargo delivery processes, like SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex mediated membrane fusion and other vesicle fusion in live cells, enable targeted delivery with high efficiency. A surrogate of SNARE machinery represents a new direction in intracellular protein delivery.Author: Lele Sun, Yanjing Gao, Yaoguang Wang, Qin Wei, Jiye Shi, Nan Chen, Di Li, Di Li, Chunhai Fan
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