Protein Delivery Into Plant Cells

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Gene Delivery into Plant Cells for Recombinant Protein ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449920/
    May 17, 2015 · Recombinant proteins are primarily produced from cultures of mammalian, insect, and bacteria cells. In recent years, the development of deconstructed virus-based vectors has allowed plants to become a viable platform for recombinant protein production, with advantages in versatility, speed, cost, scalability, and safety over the current production paradigms.Author: Qiang Chen, Huafang Lai

A method using electroporation for the protein delivery of ...

    https://www.nature.com/articles/s41598-018-38119-9
    Feb 15, 2019 · Establishment of reporter T87 cell line. Protein delivery into cells is a promising approach for biotechnology, and thus has been extensively developed in mammalian cells.Author: Yuichi Furuhata, Ayako Sakai, Tomi Murakami, Mone Morikawa, Mone Morikawa, Chikashi Nakamura, Chikas...

Nanoparticle approach to simultaneously deliver proteins ...

    https://www.nanowerk.com/spotlight/spotid=26660.php
    Sep 10, 2012 · Nanoparticle approach to simultaneously deliver proteins and DNA into plant cells (Nanowerk Spotlight) Nanotechnology is gaining significant interest in plant sciences with research focusing on investigating plant genomics and gene function as well as improving crop species. The impact on agriculture could be dramatic.

Protein Delivery, Protein Transfection - Genlantis

    http://www.genlantis.com/protein-delivery.html
    Protein Delivery / Transfection Genlantis has developed a different kind of protein delivery approach using a novel reagent called BioPORTER® protein transfection reagent.BioPORTER reagent is a unique cationic lipid mixture that is hydrated with a solution of the protein that is to be delivered.

Gene delivery into plant cells for recombinant protein ...

    https://www.ncbi.nlm.nih.gov/pubmed/26075275
    Gene delivery into plant cells for recombinant protein production. Chen Q(1), Lai H(2). Author information: (1)Center for Infectious Disease and Vaccinology, The Biodesign Institute, Arizona State University, 1001 S. McAllister Avenue, Tempe, AZ 85287, USA ; School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA.Author: Qiang Chen, Huafang Lai

(PDF) Protein Delivery into Plant Cells: Toward In vivo ...

    https://www.researchgate.net/publication/316239362_Protein_Delivery_into_Plant_Cells_Toward_In_vivo_Structural_Biology
    Apr 19, 2017 · Protein Delivery into Plant Cells: Toward In vivo Structural Biology Article (PDF Available) in Frontiers in Plant Science 8:519 · April 2017 with 120 Reads How we measure 'reads'

Direct and Indirect Visualization of ... - Plant Cell

    http://www.plantcell.org/content/29/7/1555
    Jul 01, 2017 · Visualizing effector delivery during infection is challenging due to the plant cell wall, autofluorescence, and low effector abundance. Here, we used a GFP strand system to directly visualize bacterial effectors delivered into plant cells through the type III secretion system. GFP is a beta barrel that can be divided into 11 strands.Author: Elizabeth Henry, Tania Y Toruño, Alain Jauneau, Laurent Deslandes, Gitta Laurel Coaker

Gene Delivery into Plant Cells for Recombinant Protein ...

    https://www.hindawi.com/journals/bmri/2015/932161/
    The left (LB, pink triangle) and right border (RB, pink triangle) delineate the T-DNA construct that will be transferred into plant cells by Agrobacterium. Upon delivery into plant cells, expression of Rep gene produces the Rep protein (brown cloud) that nicks the LIRs (red stars) in the T-DNA to release a single-stranded DNA molecule (between ...Author: Qiang Chen, Huafang Lai

Fast-track applications: The potential for ... - Plant Methods

    https://plantmethods.biomedcentral.com/articles/10.1186/1746-4811-1-12
    Dec 15, 2005 · In animal systems, several methods exist for the direct delivery of nucleic acids and proteins into cells for functional analysis. Until recently, these methods have not been applied to plant systems. Now, however, several preliminary reports suggest that both nucleic acids and proteins can also be delivered into plant cells by very simple, direct application. This promises to open the way for ...Author: Michael R Roberts

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