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http://cshprotocols.cshlp.org/content/2012/4/pdb.ip068619.abstract
Abstract. Polylysine and its copolymers have been extensively used as nonviral polymeric gene carriers. Although polylysine on its own is toxic to cells, when polyethylene glycol is covalently linked to polylysine, toxicity is reduced and DNA transfection efficiency is increased.
https://www.ncbi.nlm.nih.gov/pubmed/22474666
Apr 01, 2012 · Polylysine copolymers for gene delivery. Kim SW. Polylysine and its copolymers have been extensively used as nonviral polymeric gene carriers. Although polylysine on its own is toxic to cells, when polyethylene glycol is covalently linked to polylysine, toxicity is reduced and DNA transfection efficiency is increased.Author: Sung Wan Kim
https://journals.sagepub.com/doi/abs/10.1177/088391150001500202
Mar 01, 2000 · Synthesis and Evaluation of Poly(Ethylene Glycol)-Polylysine Block Copolymers as Carriers for Gene Delivery. S. Vanderkerken, T. Vanheede, V. Toncheva, E. Schacht, M. A. Wolfert, L. Seymour, and A. Urtti ... Synthesis and Evaluation of Poly(Ethylene Glycol)-Polylysine Block Copolymers as Carriers for Gene Delivery Show all authors. S ...Author: Sylvie Vanderkerken, Tom Vanheede, Veska Toncheva, Etienne Schacht, Ma Wolfert, L Seymour, A Urtti
https://pubs.acs.org/doi/10.1021/ma035650c
The development of biodegradable cationic polymers for use in somatic gene therapy is desirable because degradable polymers have the potential to overcome cellular toxicities that are related to the high charge densities of the polycationic delivery system. Therefore, to produce a biocompatible gene delivery vehicle, we have designed a novel biodegradable, high molecular weight multiblock ...Author: Malavosklish Bikram, Cheol Hee Ahn, Su Young Chae, Minhyung Lee, James W. Yockman, Sung Wan Kim
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2732109/
In the present study, silk-based block copolymers were bioengineered with poly(l-lysine) domains for gene delivery. Ionic complexes of these silk-polylysine based block copolymers with plasmid DNA (pDNA) were prepared for gene delivery to human embryonic kidney (HEK) cells.Author: Keiji Numata, Balajikarthick Subramanian, Heather A. Currie, David L. Kaplan
https://www.sciencedirect.com/science/article/pii/S0142961210013517
Polyethylenimine-grafted copolymer of poly(l-lysine) and poly ... Abstract. A major challenge in gene therapy is the development of effective gene delivery vectors with low toxicity. In the present study, linear ... -co-poly(L-lysine)-g-histidine multiblock copolymers for nonviral gene delivery. Macromolecules, 37 (2004), pp. 1903-1916.Author: Jian Dai, Seyin Zou, Yuanyuan Pei, Du Cheng, Hua Ai, Xintao Shuai
https://www.sciencedirect.com/topics/neuroscience/polylysine
Polylysine can be used to stick embryos to a slide, but embryos will stick to a polylysine coated slide poorly until after fixative is washed out. This method uses depression slides with agarose-coated wells so that embryos do not have a chance to stick to a glass slide until after the fixative is well washed out.
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