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https://scialert.net/fulltext/?doi=biotech.2008.385.402
Silicon carbide fibers: One of the recently developed methods to delivery of DNA to plants is Silicon Carbide-Mediated Transformation (SCMT). Physical and chemical characteristics of silicon carbide fibers make them capable of puncturing the cells without killing them.
https://www.ncbi.nlm.nih.gov/books/NBK215771/
Klein and colleagues (1987) discovered that naked DNA could be delivered to plant cells by “shooting” them with microscopic pellets to which DNA had been adhered. This is a crude but effective physical method of DNA delivery, especially in species such as corn, rice, and other cereal grains, which Agrobacterium does not naturally transform. Many GE plants in commercial production were initially …
http://www.biologydiscussion.com/genetics/engineering/methods-of-gene-transfer-in-plants-2-methods/10824
The term direct or vector less transfer of DNA is used when the foreign DNA is directly introduced into the plant genome. Direct DNA transfer methods rely on the delivery of naked DNA into the plant cells. This is in contrast to the Agrobacterium or vector-mediated DNA transfer which may be regarded as indirect methods.
https://www.aiche.org/resources/publications/cep/2017/april/delivering-genes-plants
Delivering Genes to Plants Conventional gene delivery techniques. Conventional methods to deliver genes to plant cells can be grouped... Biolistic particle delivery. Biolistic particle delivery was developed in 1982 by Sanford, et al. (1). Electroporation. In electroporation, strong electric field ...
https://fas.org/biosecurity/education/dualuse-agriculture/2.-agricultural-biotechnology/methods-of-gene-transfer-in-plants.html
Methods of Gene Transfer in Plants. To add a desired trait to a crop, a foreign gene (transgene) encoding the trait must be inserted into plant cells, along with a “cassette” of additional genetic material. The cassette includes a DNA sequence called a “promoter,” which determines where and when the foreign gene is expressed in the host,...
https://www.cell.com/trends/biotechnology/fulltext/S0167-7799(18)30093-3
Sep 01, 2018 · In sum, plant genetic engineering has lagged behind progress in animal systems; conventional methods of biomolecule delivery to plants remain challenged by intracellular transport through cell walls, and in turn limit plant genetic transformation efficacy.Author: Francis J. Cunningham, Natalie S. Goh, Gozde S. Demirer, Juliana L. Matos, Markita P. Landry
http://www.plantcell.org/content/28/7/1510
Jul 01, 2016 · Plant transformation encompasses two distinct consecutive steps: (1) DNA introduction into plant cells (sometimes known as transient transformation, in which transgenes have not yet integrated into the genome), and (2) integration of the introduced DNA into the plant genome …Author: Fredy Altpeter, Nathan M. Springer, Laura E. Bartley, Ann E. Blechl, Thomas P. Brutnell, Vitaly Cito...
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