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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4046008/
May 21, 2014 · Nanoparticles in gene delivery. In the field of nanomedicine, nanotechnology methods focus on formulating therapeutic biocompatible agents such as nanoparticles, nanocapsules, micellar systems, and conjugates [22,23].Nanoparticles are solid and spherical structures ranging to around 100 nm in size and prepared from natural or synthetic polymers [].Author: Solmaz Maleki Dizaj, Samira Jafari, Ahmad Yari Khosroushahi
https://www.ncbi.nlm.nih.gov/pubmed/29151352
Dec 20, 2017 · For silica nanoparticles with rambutan-, raspberry-, and flower-like morphologies composed of spike-, hemisphere-, and bowl-type subunit nanotopographies, respectively, the rambutan-like nanoparticles with spiky surfaces demonstrate the highest plasmid DNA binding capability and transfection efficacy of 88%, higher than those reported for ...Author: Hao Song, Meihua Yu, Yao Lu, Zhengying Gu, Yannan Yang, Min Zhang, Jianye Fu, Chengzhong Yu
https://pubs.acs.org/doi/abs/10.1021/jacs.7b08974
Plasmid DNA molecules with unique loop structures have widespread bioapplications, in many cases relying heavily on delivery vehicles to introduce them into cells and achieve their functions. Herein, we demonstrate that control over delicate nanotopography of silica nanoparticles as plasmid DNA vectors has significant impact on the transfection efficacy.Author: Hao Song, Meihua Yu, Yao Lu, Zhengying Gu, Yannan Yang, Min Zhang, Jianye Fu, Chengzhong Yu
https://www.sciencedirect.com/science/article/pii/S0142961213008867
Therefore, nanoparticle/plasmid complexes may be a practical and exciting mode of delivery for promoting tendon healing gene therapy. One major concern for the applications of gene vectors is the transfection efficiency to specific cells in vivo. To determine the gene transfection efficiency of the pEGFP plasmid using different types of non-viral carriers into tenocytes, sections from tendons were stained …Author: Youlang Zhou, Luzhong Zhang, Weixin Zhao, Yafang Wu, Changlai Zhu, Yumin Yang
https://pubs.acs.org/doi/full/10.1021/acsabm.8b00596
To delineate the ability of this nanoparticle–plasmid complex to exhibit an improved cellular delivery, MDA-MB-231 cells were treated with a set of plasmid and plasmid–nanoparticle complexes. Successful expression of EGFP was only observed in cells treated with the biotin-modified EGFP plasmid and a streptavidin-CD44 aptamer-nanoparticle.Author: Nathan Beals, Nithya Kasibhatla, Soumitra Basu
https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(16)38877-3
May 01, 2009 · Stereotactic neurosurgery was used to unilaterally inject either the acetate or the trifluoroacetate DNA nanoparticle formulation into the murine striatum. Using an in vivo imaging system (IVIS), we assessed the level of luciferase gene activity on days 3 and 7 post-delivery of the DNA nanoparticles. Following IVIS collection on day 7, brain ...
https://www.sciencedirect.com/science/article/pii/S0168170213003687
Jan 22, 2014 · Encapsulation and delivery of plasmid DNA by virus-like nanoparticles engineered from Macrobrachium rosenbergii nodavirus. ... To confirm that plasmid DNA was encapsulated into the inner cavity of VLP, 1 U of DNAseI was added into plasmid loaded VLP suspension to allow any exterior DNA digestion (if any) followed by treating and analyzing by ...Author: Pitchanee Jariyapong, Pitchanee Jariyapong, Charoonroj Chotwiwatthanakun, Monsicha Somrit, Sarawut J...
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