Microinjection As A Tool Of Mechanical Delivery

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Microinjection as a tool of mechanical delivery Request PDF

    https://www.researchgate.net/publication/23192075_Microinjection_as_a_tool_of_mechanical_delivery
    Microinjection as a tool of mechanical delivery Article · Literature Review in Current Opinion in Biotechnology 19(5):506-10 · October 2008 with 38 Reads How we measure 'reads'

Controlled delivery of β-globin-targeting TALENs and ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642230/
    Controlled delivery of β-globin-targeting TALENs and CRISPR/Cas9 into mammalian cells for genome editing using microinjection. ... Zhang Y. & Yu L.-C. Microinjection as a tool of mechanical delivery. Current Opinion in Biotechnology 19, 506–510 (2008).

Single-Cell Microinjection Technologies SpringerLink

    https://link.springer.com/protocol/10.1007%2F978-1-61779-567-1_13
    Single-cell microinjection, as a mechanical delivery tool, has been used for transferring substances into transfection or infection challenging cells. Here, we discuss the advantages and applications of microinjection, list the materials needed for performing microinjection experiments, and describe the methods of single-cell microinjection ...

Single-cell microinjection technologies.

    https://www.ncbi.nlm.nih.gov/pubmed/22323147
    Single-cell microinjection, as a mechanical delivery tool, has been used for transferring substances into transfection or infection challenging cells. Here, we discuss the advantages and applications of microinjection, list the materials needed for performing microinjection experiments, and describe the methods of single-cell microinjection ...

Mechanical behavior of cells in microinjection: A minimum ...

    https://www.sciencedirect.com/science/article/pii/S1751616113001409
    Mechanical behavior of cells in microinjection: A minimum potential energy study. ... Microinjection is one of the most effective methods to introduce foreign materials into living cells ... L.C. YuMicroinjection as a tool of mechanical delivery. Current Opinion in Biotechnology, 19 (2008), pp. 506-510.

Passive microinjection within high-throughput ...

    https://www.nature.com/articles/s41598-019-43056-2
    Apr 30, 2019 · Microinjection is an effective actuation technique used for precise delivery of molecules and cells into droplets or controlled delivery of genes, molecules, proteins, and viruses into single cells.

A vector-free microfluidic platform for intracellular delivery

    https://www.pnas.org/content/110/6/2082
    Intracellular delivery of macromolecules is a challenge in research and therapeutic applications. Existing vector-based and physical methods have limitations, including their reliance on exogenous materials or electrical fields, which can lead to toxicity or off-target effects. We describe a microfluidic approach to delivery in which cells are mechanically deformed as they pass through a ...

Towards Ultrahigh Throughput Microinjection: MEMS-based ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4492306/
    We report the design, fabrication, and testing of a MEMS-based device for cellular manipulation via massively parallelized mechanoporation. While low efficiencies are observed, preliminary validation of the device concept is demonstrated, thus representing a key step towards the ultimate realization of devices capable of UHT microinjection.

Towards Ultrahigh Throughput Microinjection: MEMS-based ...

    http://europepmc.org/articles/PMC4492306
    I. Introduction. Microinjection is a well-established cellular manipulation technique that enables introduction of exogenous materials into a cell [1, 2].As shown in Fig. 1, microinjection instrumentation typically requires an operator to first locate the cell to be manipulated and then capture it using aspiration (i.e. suction) from a micropipette attached to a manually-controlled ...

Microinjection for Single-Cell Analysis SpringerLink

    https://link.springer.com/chapter/10.1007/978-3-662-49118-8_4
    Jan 22, 2016 · Needle microinjection is one of them and it is widely used to modulate cellular functions by introducing foreign cargo into the cell. Microinjection is a method that can directly deliver a precise amount of foreign cargo either into the cytoplasm or the nucleus of a single cell using micropipettes. ... Microinjection as a tool of mechanical ...

(PDF) Improvised microinjection technique for mosquito vectors

    https://www.researchgate.net/publication/235421832_Improvised_microinjection_technique_for_mosquito_vectors
    Improvised microinjection technique for mosquito vectors. ... Microinjection as a tool of mechanical 1. ... viral vectors for gene delivery have been developed to surmount extracellular and ...

(PDF) A Review of Automated Microinjection of Zebrafish ...

    https://www.researchgate.net/publication/329900081_A_Review_of_Automated_Microinjection_of_Zebrafish_Embryos
    Cell microinjection is a technique of precise delivery of substances into cells and is widely used for studying cell transfection, signaling pathways, and organelle functions.

Quantitative microinjection using fluorescence calibration ...

    https://www.researchgate.net/publication/326196170_Quantitative_microinjection_using_fluorescence_calibration_of_streaming_microdroplets_on_a_superhydrophobic_surface
    Microinjection as a tool of mechanical delivery. Article. Oct 2008 ... This review discusses the advantages and limits of microinjection as a mechanical delivery method and its applications to ...

Components of a Microinjection System

    http://cshprotocols.cshlp.org/content/2011/8/pdb.ip27.full
    Direct-pressure microinjection with a micropipette is an essential tool for introducing a variety of impermeant substances into the cytoplasm or nucleus of plant and animal cells. Microinjection remains the most direct method to gain insight into the function and dynamics of intracellular components, to produce transgenic animals, or to ...

A new minimally-invasive method for microinjection into ...

    https://www.nature.com/articles/srep14306
    Sep 21, 2015 · New minimally invasive method for intra-spinal dorsal horn (SDH) microinjection. (a) Skeletal preparation of mouse vertebral column and pelvis viewed from …

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