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http://europepmc.org/patents/PAT/US2011274682
A method for intracellular delivery of single proteins or other cargo molecules by encapsulation within nanocapsules formed by interfacial polymerization of one or more types of monomers and selected protease cleavable cross-linkers is provided.
http://www.freepatentsonline.com/y2020/0009070.html
Jan 09, 2020 · FIG. 1 is a flow diagram of a method for protease-mediated intracellular protein delivery with nanocapsules according to one embodiment of the invention. FIG. 2A and FIG. 2B are schematic diagrams of alternative cross-linker synthesis schemes of one embodiment of the method for protease-mediated intracellular protein delivery.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2792531/
Enzyme inhibitors: The choice of protease inhibitors will depend on the structure of these therapeutic drugs, and the information on the specificity of proteases is essential to guarantee the stability of the drugs in the GI tract 20.The quantity of co-administered inhibitor(s) is essential for the intestinal permeability of a peptide or protein drug.
https://www.portlandpress.com/biochemsoctrans/article/doi/10.1042/BST20190039/222602/Methods-for-protein-delivery-into-cells-from
The manipulation of cultured mammalian cells by the delivery of exogenous macromolecules is one of the cornerstones of experimental cell biology. Although the transfection of cells with DNA expressions constructs that encode proteins is routine and simple to perform, the direct delivery of proteins into cells has many advantages. For example, proteins can be chemically modified, assembled into ...
https://www.sciencedirect.com/science/article/pii/S0142961216305956
Furthermore, controlled release of VEGF from degradable microgels promoted increased vascularization compared to empty microgels or bolus injection of VEGF. Collectively, this study motivates the use of microgels as a viable method for controlled protein delivery in regenerative medicine applications.Author: Greg A. Foster, Devon M. Headen, Cristina González-García, Cristina González-García, Manuel Salmerón...
https://lupinepublishers.com/agriculture-journal/fulltext/plant-based-protein-sources-and-extraction.ID.000130.php
Enzyme-assisted protein extraction (EAE) depends on disrupting cell wall and increasing yield in mild conditions. Therefore, it is more environmental-friendly method than using harsh chemicals. Specific enzymes for extraction methods have been developed for extraction of proteins from tea leaves, Leguminosae gums, oilseed meals and so on.Author: Ayten Gizem zbek, Seda Ersus Bilek
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