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https://www.sciencedirect.com/science/article/abs/pii/S0168365907006542
The delivery of functional therapeutic proteins by lipid vesicles into targeted living cells is one of the most promising strategies for treatment of different diseases and cancer. The use of this system in the delivery of membrane proteins directly into cells remains to be tested because the methods for producing membrane proteins are difficult to perform.Author: Lavinia Liguori, Bruno Marques, Ana Villegas-Mendez, Romy Rothe, Jean-Luc Lenormand
https://www.researchgate.net/publication/5618317_Liposomes-mediated_delivery_of_pro-apoptotic_therapeutic_membrane_proteins
Liposomes-mediated delivery of pro-apoptotic therapeutic membrane proteins. The delivery of functional therapeutic proteins by lipid vesicles into targeted living cells is one of the most promising strategies for treatment of different diseases and cancer.
https://www.nature.com/articles/srep18025
Dec 15, 2015 · Therefore, we performed a comprehensive study to evaluate the effects of liposomes on 85 aggregation-prone membrane proteins from Escherichia coli by using a reconstituted, chemically defined cell-free translation system. Statistical analyses revealed that the presence of liposomes increased the solubility of >90%...Author: Tatsuya Niwa, Yoshihiro Sasaki, Eri Uemura, Shugo Nakamura, Minato Akiyama, Mitsuru Ando, Shinichi S...
https://onlinelibrary.wiley.com/doi/full/10.1002/adfm.201303222
Gel–Liposome‐Mediated Co‐Delivery of Anticancer Membrane‐Associated Proteins and Small‐Molecule Drugs for Enhanced Therapeutic Efficacy Tianyue Jiang Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, NC, 27695 USAAuthor: Tianyue Jiang, Tianyue Jiang, Ran Mo, Ran Mo, Adriano Bellotti, Jianping Zhou, Zhen Gu
https://www.sciencedirect.com/journal/journal-of-controlled-release/vol/126/issue/3
Mar 20, 2008 · Journal of Controlled Release. ... select article Liposomes-mediated delivery of pro-apoptotic therapeutic membrane proteins. ... Research article Full text access Liposomes-mediated delivery of pro-apoptotic therapeutic membrane proteins. Lavinia Liguori, Bruno Marques, Ana Villegas-Mendez, Romy Rothe, Jean-Luc Lenormand ...
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/pro-apoptotic-proteins
The delivery of pro-apoptotic protein, TRAIL, via SC offers a promising on-site delivery approach toward tumor cell killing [17]. TRAIL is an endogenous member of the TNF ligand family that binds to its death domain containing receptors Dr4 and Dr5 and induces apoptosis via activation of caspases, preferentially in cancer cells while sparing most other cell types [125] .
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4678891/
Translation of membrane proteins in the presence of liposomes as an artificial cell membrane seems to be straightforward and attractive approach for cell-free systems. Several reports have described successful cell-free expression of several membrane proteins in the presence of liposomes.Author: Tatsuya Niwa, Yoshihiro Sasaki, Eri Uemura, Shugo Nakamura, Minato Akiyama, Mitsuru Ando, Shinichi S...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3090341/
Apr 11, 2011 · Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system ... Villegas-Méndez A, Rothe R, Lenormand JL. Liposome-mediated delivery of pro-apoptotic therapeutic membrane proteins. J Cont Rel ... A Protein Structure Initiative approach to expression, purification, and in situ ...Author: Akira Nozawa, Tomio Ogasawara, Satoko Matsunaga, Takahiro Iwasaki, Tatsuya Sawasaki, Yaeta Endo
https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-11-35
Apr 11, 2011 · (D) The relationship between the extent of association and solubility of each protein. Membrane and soluble proteins were synthesized using the wheat cell-free system supplemented with or without liposomes and [14 C]-leucine. Proteins synthesized without liposomes were separated into soluble and insoluble fraction by centrifugation.Author: Akira Nozawa, Tomio Ogasawara, Satoko Matsunaga, Takahiro Iwasaki, Tatsuya Sawasaki, Yaeta Endo
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