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https://journals.lww.com/anesthesia-analgesia/pages/articleviewer.aspx?year=2010&issue=08000&article=00039&type=Fulltext
Twenty-four hours after the last preconditioning day, bilateral common carotid artery occlusion was performed as a model of global cerebral ischemia for 20 minutes in the IsoPC group and Con group.
https://pubmed.ncbi.nlm.nih.gov/22103751/
Results: Two to three hours of reperfusion were required for recovery of WT following either ISOPC or IPC; in contrast, without PC, WT remained markedly reduced (30% below baseline) at this time point and required more than 6 h of reperfusion for recovery. Neither IPC nor ISOPC affected expression of Cu-Zn SOD, Mn SOD, or catalase.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2266686/
Twenty-four hours after preconditioning (sham PC, IsoPC), young male and female C57BL/6 mice were euthanized. Cortices were subdissected from the left hemisphere of preconditioned mouse brains (n = 7 per treatment group, total = 28), frozen in 2-methylbutane at −30°C, and stored at −80°C until analyzed. For TaqMan qPCR measurement of NIPK mRNA, total RNA was isolated using the RNeasy Mini kit …
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