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https://www.medicobio.com/product-page/hypoxyprobe-plus-kit-100-mg-pimonidazole-hcl-plus-1-unit-of-4-3-11-3-mouse-fitc#:~:text=The%20plasma%20half-life%20of%20Hypoxyprobe%E2%84%A2-1%20is%200.5%20hours,skin%20have%20cells%20at%2C%20or%20below%2C%20this%20pO2.
http://site.hypoxyprobe.com/knowledge-center-articles/HP18-Hypoxyprobe-Times-Kit-Insert-2020.pdf
Typically, cell suspensions are incubated under hypoxia for 1 to 2 hours in the presence of 100 to 200 micromolar Hypoxyprobe™-1. The cells are harvested by cytospin, fixed and
http://www.hypoxyprobe.com/faq.html
Exposure = 58mg/kg x 1 hour = 58mg/kg-hour. Strong staining for hypoxia in tumor tissue and in normal epithelia is obtained in humans with a dose of 0.5gm/m2 (ca 14mg/kg) where the plasma half-life of pimonidazole is 5 hours. Exposure = 14 mg/kg x 5 hours = 70 mg/kg-hour.
http://site.hypoxyprobe.com/knowledge-center-articles/HP-1-Kit-Insert.pdf
In addition to animal studies, Hypoxyprobe™ kits can be used for cells in tissue culture (see Applications link at www.hypoxprobe.com). Typically, cell suspensions are incubated under hypoxia for 1 to 2 hours in the presence of 100 to 200 micromolar Hypoxyprobe™-1. The cells are then
http://site.hypoxyprobe.com/hypoxyinfo.htm
The plasma half-life of Hypoxyprobe is 0.5 hours in C3H/He mice. Hypoxyprobe is distributed to all tissues in the body including brain but binds only to cells that have oxygen concentrations less than 14 micromolar which is equivalent to a pO 2 of 10 mm Hg at 37 o C. Tumors and normal liver, kidney and skin have cells at, or below, this pO 2 .
http://site.hypoxyprobe.com/faq.htm
In the initial human tumor studies, biopsies were taken 16-24 hours after Hypoxprobe infusion .The plasma half-life of Hypoxyprobe-1 in humans is ca 5 hours so that 16 to 24 hours represents 3 to 5 half-lives of circulating marker.This means that 1/8 to 1/32 of the initial concentration of marker is present at the time of harvesting. This, combined with rapid transfer of biopsy material to cold fixative, minimized …
http://www.hypoxyprobe.com/
We hope you enjoy the new and improved Hypoxyprobe Website with its new features and ease of use. Please visit the Knowledge Center for details of how Hypoxyprobe Kits detect tissue hypoxia and references to their application in studies of hypoxia in normal and tumor tissues in a range of species by a variety of detection techniques (immunoperoxidase, immunofluorescence, flow cytometry, etc).
https://www.bioz.com/result/hypoxyprobe%201%20kit/product/Millipore
Figure Legend Snippet: A: Hypoxyprobe staining. Representative murine endometriotic tissue removed at different time points after transplantation. D: 1 hour. E: 4 hours. F: 24 hours. G: 48 hours. H: 1 week. I: 6 months. Each mouse was injected with pimonidazole hydrochloride
https://www.medicobio.com/product-page/hypoxyprobe-plus-kit-100-mg-pimonidazole-hcl-plus-1-unit-of-4-3-11-3-mouse-fitc
The plasma half-life for Hypoxyprobe™-1 in dogs is 1.5 ± 1.0 hours. In addition to animal studies, Hypoxyprobe™ kits can be used for cells in tissue culture (6, 44). Typically, cell suspensions are incubated under hypoxia for 1 to 2 hours in the presence of 100 to 200 micromolar Hypoxyprobe™-1.
http://site.hypoxyprobe.com/knowledge-center-articles/Hypoxyprobe_Red_594_Kit.pdf
specific binding due to residual Hypoxyprobe™-1 is undetectable. In addition to animal studies, Hypoxyprobe™ kits can be used for cells in tissue culture (see Applications link at www.hypoxyprobe.com). Typically, cell suspensions are incubated under hypoxia for 1 to 2 hours in the presence of 100 to 200 micromolar Hypoxyprobe™-1.
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