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https://www.frontiersin.org/articles/10.3389/fcell.2018.00072/full#:~:text=In%20vitro%20incubation%20under%20capacitating%20conditions%20for%20human,using%201%E2%80%931.5%20h%20of%20incubation%20under%20capacitating%20conditions.
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/sperm-capacitation
Sperm capacitation represents the culmination of a spermatozoon’s ... and sperm and oocytes are coincubated between 16 and 24 hours at 38 ... sperm or to a nonfunctional receptor. 53 Meizel and co-authors have studied the progesterone-induced acrosome reaction in human sperm 37,54 and in porcine sperm, 55 and they have demonstrated that ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6078053/
In vitroincubation under capacitating conditions for human sperm ranges from 3 to 24 h. As a result, a great variability of results is reported in the literature. In contrast, most studies in mouse sperm are performed using 1–1.5 h of incubation under capacitating conditions.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720105/
Ejaculated mammalian spermatozoa should reside in the female genital tract for several hours before gaining the ability to fertilize the egg. In humans however, sperm must move out of the seminal plasma immediately after ejaculation and appear in …
https://pubmed.ncbi.nlm.nih.gov/19408963/
This work deals with a proteomic approach to the analysis of protein profile variations in human normospermic samples as a consequence of three hours in vitro capacitation. 2DE gels were produced per freshly ejaculated sperm and per capacitated sperm and several quantitative and qualitative significant variations were found.
https://www.frontiersin.org/articles/10.3389/fcell.2018.00072/full
However, in humans, the timing of when sperm encounter the egg is spread over a long period of time (2–3 days), and concomitantly, human sperm require long incubation time (more than 3 h) to undergo capacitation-related events.
https://pubmed.ncbi.nlm.nih.gov/30105226/
In the early 1950s, Austin and Chang independently described the changes that are required for the sperm to fertilize oocytes in vivo.These changes were originally grouped under name of "capacitation" and were the first step in the development of in vitro fertilization (IVF) in humans. Following these initial and fundamental findings, a remarkable number of observations led to …
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