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https://www.thermofisher.com/order/catalog/product/AM4626
Ambion® Silencer® GFP (eGFP) Positive Control siRNA is ideal for developing and optimizing siRNA experiments. 5 nmol is provided, along with an additional tube of 2 nmol negative control siRNA. • Validated siRNA controls for optimizing siRNA experiments • Gene-specific control siRNAs provided with scrambled, negative controls
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4894740/
Jul 22, 2015 · An alternate anti-Gfp siRNA sequence (Seq. 2) was produced based on complementarity to the Gfp sequence. NSP delivery of anti-Gfp siRNA (sequence 2) did not produce knockdown (Figure 6). The nanogel 1:1 and 10:1 ratios were not effective against GFP expression (n = 8, p = 0.567 and p = 0.428, respectively). This is a strong indicator that ...Author: Arun R. Shrivats, Yuji Mishina, Saadyah Averick, Krzysztof Matyjaszewski, Jeffrey O. Hollinger
https://www.addgene.org/12273/
Plasmid pLKO.1 puro GFP siRNA from Dr. Bob Weinberg's lab contains the insert GFP siRNA and is published in Cell. 2005 May 6. 121(3):335-48. This plasmid is available through Addgene.
https://www.nature.com/articles/nm1486
Feb 25, 2007 · The synthesis of the GFP-targeting complex, MN-NIRF-siGFP, resulted in triple-labeled nanoparticles that had on average three Cy5.5, four MPAP and five siRNA molecules per …Author: Zdravka Medarova, Wellington Pham, Christian Farrar, Victoria Petkova, Anna Moore
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4150469/
Mar 24, 2014 · We report hypoxia-activated green fluorescent protein (GFP) silencing in vitro and its downregulation in GFP expressing tumors after intravenous administration. The proposed nanoformulation represents a novel tumor environment-responsive modality for cancer targeting and siRNA delivery.Author: Federico Perche, S. Biswas, T. Wang, L. Zhu, L. Zhu, V. Torchilin
http://www.nature.com/articles/nbt1101
May 22, 2005 · Delivery of Ku70 siRNA (1,000 pmol) by ML39 scFv-P, but not using control proteins or ML39 scFv-P plus GFP siRNA, silences Ku70. MFI, mean fluorescence intensity. ( c ) …Author: Erwei Song, Pengcheng Zhu, Sang Kyung Lee, Dipanjan Chowdhury, Steven Kussman, Derek M Dykxhoorn, Yi...
https://www.sciencedirect.com/science/article/pii/S0378517319308476
1. Introduction. The delivery of RNA molecules (i.e. short interfering RNA (siRNA), microRNA (miRNA), short hairpin RNA (shRNA)) to silence aberrant expression of genes in a cell is a potentially powerful therapeutic strategy for a variety of diseases and cancer in recent decades.Inducing RNA interference (RNAi) (Ruigrok et al., 2016) by the delivery of exogenous small RNA molecules as a ...Author: Yong-Yu Jhan, Daniel Prasca-Chamorro, Guillermo Palou Zuniga, David Mitchell Moore, Shreedevi Arun K...
https://www.thermofisher.com/us/en/home/references/ambion-tech-support/rnai-sirna/tech-notes/sirna-expression-vectors--with-selectable-markers.html
To demonstrate the use of Ambion's new selectable marker containing pSilencer siRNA expression vectors, HeLa cells expressing GFP were transfected with pSilencer 2.1-U6 hygro encoding an siRNA to GFP and selected with the antibiotic hygromycin (Figure 2). Three weeks after transfection, GFP levels were analyzed by fluorescence microscopy.
https://www.scbt.com/p/gfp-sirna-a-victoria-shrna-and-lentiviral-particle-gene-silencers
shRNA sequences correspond to GFP siRNA Gene Silencer sequences After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target ...
https://ibidi.com/img/cms/support/AN/AN54_Fuse-It-siRNA_GFP_Knockdown.pdf
In this Application Note, the knockdown of GFP by siRNA transfer into transient GFP-expressing CHO-K1 cells is described. For the delivery of siRNA, three different reagents were compared: Fuse-It-siRNA, Lipofectamine RNAiMAX, and METAFECTENE SI+. Additionally, a control experiment without the delivery of siRNA was carried out.
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