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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2811526/
iPS cell technology is still in its infancy. In this review article, we present a comprehensive survey of reprogramming approaches focusing on gene-delivery systems used for generation of iPS cells from somatic cells, categorize gene-delivery vectors, and discuss their advantages and limitations for somatic cell reprogramming.Author: Lijian Shao, Wen Shu Wu
https://link.springer.com/chapter/10.1007/978-981-10-1560-1_11
Dec 10, 2016 · Each gene delivery and expression system has particular advantages and disadvantages depending on the desired outcome of the experimental design. Here, we briefly review representative gene delivery and expression systems from the perspective of their application to iPS cell research.Author: Maolin Zhang, Kunimichi Niibe, Takeru Kondo, Yuya Kamano, Makio Saeki, Hiroshi Egusa
https://www.takarabio.com/learning-centers/gene-function/viral-transduction/recombinant-virus-comparison
Viral gene delivery systems Viral gene delivery allows the introduction and expression of a gene of interest in almost any mammalian cell type. However, selecting the best viral gene delivery system for your experimental aim can be challenging.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379519/
Feb 18, 2019 · Gene transduction options for pluripotent stem cells are most commonly divided into two groups: viral and non-viral gene delivery (further subdivided into chemical or physical gene delivery methods). Viral delivery exploits the backbone of a viral genome with insertion of the gene or genes of interest to be expressed in the stem cell.
https://geneva-biotech.com/
Geneva Biotech Welcomes You! Geneva Biotech is a privately held biotechnology company, based in Geneva, Switzerland. We are a research focused SME with a vision to develop a portfolio of viral and nonviral DNA delivery systems that provide new and better ways to deliver DNA into cells.
https://www.mdpi.com/1999-4915/11/1/28/pdf
2. Viral Delivery Systems Many viral delivery systems have been used for delivery of CRISPR-Cas9. A brief summary of the viral vectors is shown in Table1. Table 1. Summary of different delivery vectors. Packaging Capacity Genetic Material Vector Genome Form Key Features AAV 4.7 kb ssDNA Mainly episomal Gene augmentation therapy of small genes ...Author: Christine L. Xu, Merry Z. C. Ruan, Vinit B. Mahajan, Stephen H. Tsang
https://www.sciencedirect.com/science/article/pii/S174067490800005X
Here, we assess the benefits and potential disadvantages of commonly used gene delivery systems for application to stem cell biology and iPS cell reprogramming. Gene delivery systems are varied (see Table 1) and substantial and the breadth of viral and non-viral technologies has developed at a great pace during the past 15 years within the gene ...Author: Nicole M. Kane, Scott McRae, Chris Denning, Andrew H. Baker
https://www.takarabio.com/learning-centers/gene-function/gene-editing/crispr/cas9-delivery-methods
For gene editing in mammalian cells we typically recommend delivery of a ribonuclear protein (RNP) complex consisting of Cas9 protein and a single guide RNA (sgRNA). RNPs remain in the cell for a short time and the dose is minimal, leading to lower toxicity and …
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