Electroporation Gene Delivery

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Electroporation-Mediated Gene Delivery - ScienceDirect

    https://www.sciencedirect.com/science/article/abs/pii/S0065266014000042
    Electroporation has been used extensively to transfer DNA to bacteria, yeast, and mammalian cells in culture for the past 30 years. Over this time, numerous advances have been made, from using fields to facilitate cell fusion, delivery of chemotherapeutic drugs to cells and tissues, and most importantly, gene and drug delivery in living tissues from rodents to man.Author: Jennifer L. Young, David A. Dean

Gene Delivery Technologies Thermo Fisher Scientific - US

    https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/transfection-basics/gene-delivery-technologies.html
    Gene Delivery Technologies Cationic Lipid Transfection Calcium Phosphate Transfection DEAE-Dextran Transfection Other Cationic Polymers Viral Transfection Electroporation Other Physical Delivery Methods Factors Influencing Transfection Efficiency ›

Electroporation - an overview ScienceDirect Topics

    https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/electroporation
    Electroporation can lead to between 100 and 1000-fold increases in gene delivery and expression and can also increase both the distribution of cells taking up and expressing the DNA as well as the absolute amount of gene product per cell (likely due to increased delivery of plasmids into each cell).

Electroporation-enhanced gene delivery in mammary tumors ...

    https://www.nature.com/articles/3301141
    Apr 12, 2000 · Electroporation was applied to enhance gene transfer into subcutaneous MC2 murine breast tumors. Cultured MC2 cells were also transfected …Author: J M Wells, L H Li, A Sen, G P Jahreis, S W Hui

Electroporation – Advantages and Drawbacks for Delivery of ...

    https://www.intechopen.com/books/application-of-nanotechnology-in-drug-delivery/electroporation-advantages-and-drawbacks-for-delivery-of-drug-gene-and-vaccine
    Electroporation was introduced in the 1960s and comprises the application of controlled electric fields to facilitate cell permeabilization. The success of in vitro delivery by electroporation has led to the development of in vivo applications [Takei et al., 2008].The first in vitro and in vivo attempts to use electroporation in gene transfer were demonstrated in 1982 and 1991, respectively ...Author: Azam Bolhassani, Afshin Khavari, Zahra Orafa

Retinal gene delivery by rAAV and DNA electroporation

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3643812/
    This unit describes the in vivo delivery of genes to the mouse eye by rAAV-mediated gene transfer and plasmid DNA electroporation. Advantages and limitations of these methods are discussed, and detailed protocols for gene delivery, required materials, as well as …Author: Aditya Venkatesh, Shan Ma, Fernanda Langellotto, Fernanda Langellotto, Guangping Gao, Claudio Punzo

Highly efficient gene delivery by mRNA electroporation in ...

    https://ashpublications.org/blood/article/98/1/49/88901/Highly-efficient-gene-delivery-by-mRNA
    Jul 01, 2001 · Highly efficient gene delivery by mRNA electroporation in human hematopoietic cells: superiority to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for tumor antigen loading of dendritic cellsAuthor: Viggo F. I. Van Tendeloo, Peter Ponsaerts, Filip Lardon, Griet Nijs, Marc Lenjou, Christine Van Broe...

Electroporation Delivery of DNA Vaccines: Prospects for ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3109217/
    Electroporation Enhanced DNA Vaccine Delivery. Electroporation (EP) is a method to introduce macromolecules such as nucleic acids into cells, either in vivo or in vitro, via the application of brief electric pulses to induce transient and reversible permeabilization of the cell membrane (Figure 1).Author: Niranjan Y Sardesai, David B Weiner

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