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https://pubs.acs.org/doi/10.1021/cb600127m
However, the addition of pyrenebutyrate results in direct membrane translocation of the peptides yielding diffuse cytosolic peptide distribution within a few minutes. Using this method, rapid and efficient cytosolic delivery of the enhanced green fluorescent protein (EGFP) was achieved in cells including rat hippocampal primary cultured neurons.Author: Toshihide Takeuchi, Michie Kosuge, Akiko Tadokoro, Yukio Sugiura, Mayumi Nishi, Mitsuhiro Kawata, Na...
https://www.ncbi.nlm.nih.gov/pubmed/17163758
Jun 20, 2006 · However, the addition of pyrenebutyrate results in direct membrane translocation of the peptides yielding diffuse cytosolic peptide distribution within a few minutes. Using this method, rapid and efficient cytosolic delivery of the enhanced green fluorescent protein (EGFP) was achieved in cells including rat hippocampal primary cultured neurons.Author: Toshihide Takeuchi, Michie Kosuge, Akiko Tadokoro, Yukio Sugiura, Mayumi Nishi, Mitsuhiro Kawata, Na...
https://www.researchgate.net/publication/6635628_Direct_and_Rapid_Cytosolic_Delivery_Using_Cell-Penetrating_Peptides_Mediated_by_Pyrenebutyrate
Using this method, rapid and efficient cytosolic delivery of the enhanced green fluorescent protein (EGFP) was achieved in cells including rat hippocampal primary cultured neurons.
https://www.sciencedirect.com/science/article/pii/S0168365912005792
Direct membrane translocation, an alternative mode of entry, leads to efficient cytosolic delivery of the CPP, but it only occurs above a threshold concentration that is unique to each CPP and cell type [25], [41]. Moreover, although increasing the CPP concentration may enhance the cytosolic distribution …Author: Yan Ma, Cheng Gong, Yilong Ma, Fengkai Fan, Meijie Luo, Fei Yang, Yu-Hui Zhang
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757120/
Aug 27, 2013 · Protein-based biologics have provided new therapeutic avenues for cancer, 2,3 and have also been used to treat a range of disease states including inflammation, 4 lysosomal storage diseases, 5 and transient cerebrovascular disorders. 6 In addition to therapeutics, direct cytosolic delivery of functional proteins provides a potential tool for important biological applications including imaging, 7 signaling studies, 8,9 cellular 10, 11 and stem cell engineering…Author: Rui Tang, Chang Soo Kim, David J. Solfiell, Subinoy Rana, Rubul Mout, Elih M. Velázquez-Delgado, Api...
https://onlinelibrary.wiley.com/doi/full/10.1002/anie.200903112
Nov 11, 2009 · Getting straight to the point: A peptide–phospholipid‐based biomimetic nanocarrier was developed that can transport cargo molecules directly into cytosolic compartments through a non‐endocytotic uptake mechanism mediated by scavenger receptor class B type I (SR‐BI; see schematic illustration). The fluorescent image shows the cytosolic localization of a dye (red) delivered by the …Author: Zhihong Zhang, Zhihong Zhang, Weiguo Cao, Weiguo Cao, Honglin Jin, Honglin Jin, Jonathan F. Lovell, ...
https://www.researchgate.net/publication/328723871_Bypassing_Endocytosis_Direct_Cytosolic_Delivery_of_Proteins
Bypassing Endocytosis: Direct Cytosolic Delivery of Proteins. Therapeutic proteins have increased dramatically in both number and frequency of use in recent years, primarily owing to advances in protein engineering. Protein therapy provides the advantages of high potency and specificity, as …
https://onlinelibrary.wiley.com/doi/full/10.1002/anie.201409161
Abstract. The use of nanoparticle‐stabilized nanocapsules (NPSCs) for the direct cytosolic delivery of siRNA is reported. In this approach, siRNA is complexed with cationic arginine‐functionalized gold nanoparticles by electrostatic interactions, with the resulting ensemble self‐assembled onto the surface of fatty acid nanodroplets...Author: Ying Jiang, Rui Tang, Bradley Duncan, Ziwen Jiang, Bo Yan, Rubul Mout, Vincent M. Rotello
https://www.sciencedirect.com/science/article/pii/S0142961219304570
For example, there is a rapidly increasing need for the direct delivery of endonuclease such as clustered regularly interspaced short palindromic repeats (CRISPR) associated protein 9 (Cas9), zinc finger nucleases (ZFN) and recombinase such as cyclization recombinase (CRE) into cells for genome editing [, , , , , , ]. Therefore, increasing attentions were paid to develop facile and efficient techniques for cytosolic protein delivery …Author: Jia Lv, Qianqian Fan, Hui Wang, Yiyun Cheng, Yiyun Cheng
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