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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3507026/
Jul 06, 2012 · Physical methods of nonviral gene delivery. Physical methods applied for in vitro and in vivo gene delivery are based on making transient penetration in cell membrane by mechanical, electrical, ultrasonic, hydrodynamic, or laser-based energy so that DNA entrance into the targeted cells is facilitated.
https://www.sciencedirect.com/science/article/pii/S0889858805700046
Direct DNA Delivery. Of the major methods of nonviral, mediated gene transfer, direct DNA delivery is probably the simplest, because it involves injection of a plasmid DNA containing a transgene into an organ site of choice.Author: Nelson A. Wivel, James M. Wilson
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722627/
Progress in molecular biology and especially, molecular medicine is now changing the basics of clinical medicine. A variety of viral and non-viral possibilities are available for basic and clinical research. This review summarizes the delivery routes and methods for gene transfer used in gene therapy.
https://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/transfection-support/gene-delivery-selection-guide.html
Researchers have a wide variety of gene delivery techniques to introduce plasmid DNA, siRNA or duplex RNAi, oligonucleotides, and RNA into eukaryotic cells for a …
https://www.sciencedirect.com/science/article/abs/pii/S0889858805700046
One of the most important characteristics is the ability of the virus to assure transport of its genomic DNA to the nucleus of the host cell without degradation by lysosomes. 45 It has become clear that one can take advantage of some of the barriers to gene expression by using viruses as the delivery vehicles, and this is discussed in more ...Author: Nelson A. Wivel, James M. Wilson
https://www.takarabio.com/learning-centers/gene-function/gene-editing/crispr/cas9-delivery-methods
For gene editing in mammalian cells we typically recommend delivery of a ribonuclear protein (RNP) complex consisting of Cas9 protein and a single guide RNA (sgRNA). RNPs remain in the cell for a short time and the dose is minimal, leading to lower toxicity and …
http://lib.store.yahoo.net/lib/yhst-131428861332406/How-to-Choose-the-Optimal-Gene-Delivery-Method.pdf
How To Choose the Optimal Gene Delivery Method Contents Page 1. Introduction 2 2. The Early Development of Facilitated Gene Delivery 2 ... in the 1970's, the existing gene delivery methods helped open a new era in biology that led to ... in different cell types compared to cationic lipids.
http://www.eagri.org/eagri50/GPBR311/lec23.pdf
Gene delivery methods in plants For production of transgenic animals, DNA is usually microinjected into pronuclei of embryonic cells at a very early stage after fertilization, or alternatively gene targeting of embryo stem (ES) cells is employed. This is possible in animals due to the
https://www.intechopen.com/books/gene-therapy-tools-and-potential-applications/non-viral-delivery-systems-in-gene-therapy
The simplicity is one of the characteristics of these methods. The genetic material is introduced into cells without formulating in any particulate or viral system. In a recent publication, Kamimura et al. revised the different physical methods for gene delivery. These methods include the following:Author: Alicia Rodríguez Gascón, Ana del Pozo-Rodríguez, María Ángeles Solinís
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