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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679364/
While the delivery of siRNAs can be achieved in many cell types, variable transfection efficiencies have limited siRNA-mediated RNAi to only those cells capable of transfection. Another form of RNAi involves the use of short hairpin RNAs (shRNAs) synthesized within the cell by DNA vector-mediated production.Author: Chris B. Moore, Elizabeth H. Guthrie, Max Tze-Han Huang, Debra J. Taxman
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949849/
Sep 21, 2010 · Selective gene silencing by RNAi can be achieved essentially by two nucleic acid based methods: i) cytoplasmic delivery of short double-stranded (ds) interfering RNA oligonucleotides (siRNA), where the gene silencing effect is only transient in nature, and possibly not suitable for all applications; or ii) nuclear delivery of gene expression ...Author: Katja Sliva, Barbara S Schnierle
https://www.researchgate.net/publication/46392655_Selective_gene_silencing_by_viral_delivery_of_short_hairpin_RNA
Selective gene silencing by viral delivery of short hairpin RNA Article · Literature Review (PDF Available) in Virology Journal 7(1):248 · September 2010 with 81 Reads How we measure 'reads'
https://www.dovepress.com/novel-guanidinylated-bioresponsive-polyamidoamines-designed-for-short--peer-reviewed-article-IJN
In order to characterize these two Gua-SS-PAA polymers and investigate their potentials as short hairpin RNA (shRNA)-delivery carriers, pSilencer 4.1-CMV FANCF shRNA was chosen as a model plasmid DNA to form complexes with these two polymers. The Gua-SS-PAAs and plasmid DNA complexes were determined with particle sizes less than 90 nm and ...Author: Jiankun Yu, Jinmin Zhang, Haonan Xing, Yanping Sun, Zhen Yang, Tianzhi Yang, Cuifang Cai, Xiaoyun Zh...
https://ashpublications.org/blood/article/106/3/818/21843/Lentiviral-delivery-of-short-hairpin-RNAs-protects
Aug 01, 2005 · Sang-Kyung Lee, Derek M. Dykxhoorn, Priti Kumar, Shahin Ranjbar, Erwei Song, Laura E. Maliszewski, Vanessa François-Bongarçon, Anne Goldfeld, N. Manjunath Swamy, Judy Lieberman, Premlata Shankar; Lentiviral delivery of short hairpin RNAs protects CD4 T cells from multiple clades and primary isolates of HIV.Author: Sang Kyung Lee, Derek M Dykxhoorn, Priti Kumar, Shahin Ranjbar, Erwei Song, Laura E. Maliszewski, Va...
https://www.sciencedirect.com/topics/medicine-and-dentistry/short-hairpin-rna
Short Hairpin RNA. Short hairpin RNA (shRNA) is an alternative way to prepare siRNA sequences for delivery to cells that can be expressed in situ from plasmid DNA (pDNA) or from virus-derived constructs. The effectiveness of shRNA was first reported by Paddison and Hannon in 2002 [48].
https://www.nature.com/articles/s41598-018-23751-2
Apr 03, 2018 · Lentiviral vector delivery of short hairpin RNA to NgR1 promotes nerve regeneration and locomotor recovery in injured rat spinal cord. Skip to main content. Thank you for visiting nature.com. ...Author: Xiaoyang Zhao, Zhiming Peng, Lingli Long, Ningning Chen, Haichong Zheng, David Y. B. Deng, Yong Wan
https://www.sciencedirect.com/science/article/pii/S0168165611004494
Inhibition of xenograft tumor growth in mice by gold nanoparticle-assisted delivery of short hairpin RNAs against Mcl-1L ... The shRNA was synthesized from synthetic oligonucleotides containing the T7 promoter followed by the sequence of a short hairpin structure of Mcl-1L or p53, as previously ... Delivery of the cy3-labeled-shRNA-Mcl-1L was ...Author: Sang-Mi Ryou, Mira Park, Jong-Myung Kim, Che Ok Jeon, Cheol-Hui Yun, Seung Hyun Han, Si Wouk Kim, Yo...
https://www.labome.com/method/siRNAs-and-shRNAs-Tools-for-Protein-Knockdown-by-Gene-Silencing.html
While both siRNAs and shRNAs (Figure 1) can be used for protein knockdown, there are differences in their mechanisms of action (Figure 2). Either long dsRNAs or short duplexes of about 21 base pairs (bps) can be introduced directly into cells in tissue culture (see Mechanisms of Delivery for further details).Author: Erin P O’Keefe
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