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https://link.springer.com/protocol/10.1385%2F1-59745-151-7%3A119
Abstract. This chapter describes four methods for delivery of double-stranded RNA to Caenorhabditis elegans (injection, feeding, soaking, and in vivo delivery), and suggests schemes that should facilitate detection of specific gene silencing.Author: Lisa Timmons
https://www.researchgate.net/publication/6806855_Timmons_L_Delivery_methods_for_RNA_interference_in_C_elegans_Methods_Mol_Biol_351_119-125
Download Citation Timmons, L. Delivery methods for RNA interference in C. elegans. Methods Mol. Biol. 351, 119-125 This chapter describes four methods for delivery of double-stranded RNA to ...Author: Lisa Timmons
https://www.sciencedirect.com/science/article/pii/S0076687904920034
RNA interference (RNAi) was first discovered in the nematode Caenorhabditis elegans (Fire et al., 1998; Guo and Kemphues, 1995).The completion of the C. elegans genome in 1998 coupled with the advent of RNAi techniques to knock down gene function ushered in a new age in the field of functional genomics. There are four methods for double-stranded RNA (dsRNA) delivery in C. elegans: (1 ...Author: Juan Wang, Maureen M. Barr
https://www.researchgate.net/publication/8087599_RNA_Interference_in_Caenorhabditis_elegans
RNA interference (RNAi) was first discovered in the nematode Caenorhabditis elegans (Fire et al., 1998; Guo and Kemphues, 1995). The completion of the C. elegans genome in 1998 coupled with the ...
https://umassmed.edu/globalassets/mellolab/documents/publications/conteprotocol2015.pdf
RNA Interference in Caenorhabditis UNIT 26.3 elegans Darryl Conte, Jr.,1 Lesley T. MacNeil,2 Albertha J.M. Walhout,2 and Craig C. Mello1,3 1RNA Therapeutics Institute and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 2Programs in Systems Biology and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5112363/
Nov 17, 2016 · Introduction. Over two and a half decades ago, the silencing ability of antisense RNA was first described in the nematode Caenorhabditis elegans (Fire et al., 1991).The interfering mediator was afterwards determined as being a double-stranded RNA (dsRNA), rather than a single-stranded antisense RNA (Fire et al., 1998).The phenomenon of RNA interference (RNAi) as a method for gene silencing …Author: Mallikarjuna R. Joga, Moises J. Zotti, Guy Smagghe, Olivier Christiaens
https://mdibl.org/wp-content/uploads/2016/11/Zhuang-and-Hunter-2011-RNA-interference-in-Caenorhabditis-elegans-Uptake.pdf
In contrast, RNAi works very well in C. elegans because of ease of delivery coupled to efficient RNA-directed RNA polymerase (RdRP) amplification of effector siRNAs. Here we review what is known about RNA delivery and genetic control of RNAi efficacy in C. elegans with the goal of using this knowledge to enable RNAi in other organisms.
https://www.ncbi.nlm.nih.gov/pubmed/15644174
RNA interference (RNAi) was first discovered in the nematode Caenorhabditis elegans (Fire et al., 1998; Guo and Kemphues, 1995). The completion of the C. elegans genome in 1998 coupled with the advent of RNAi techniques to knock down gene function ushered in a new age in the field of functional genomics.Author: Juan Wang, Maureen M. Barr
https://en.wikipedia.org/wiki/RNA_interference_machinery
RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Historically, RNAi was known by other names, including co-suppression, post-transcriptional gene silencing (PTGS), and quelling.The detailed study of each of these seemingly different processes elucidated that the identity of these phenomena ...
http://labs.bio.unc.edu/Goldstein/DudleyGoldsteinMMB05.pdf
4 RNA Interference in Caenorhabditis elegans Nathaniel R. Dudley and Bob Goldstein 1. Introduction The introduction of double-stranded RNA (dsRNA) into Caenorhabditis elegans hermaphrodites results in the rapid and sequence-specific degradation of endogenous mRNAs (1,2).This RNA-mediated interference, or …
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