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http://www.jbc.org/content/282/29/21349.full
Jul 20, 2007 · Crotamine-mediated DNA delivery studies were performed in living CHO cells to avoid fixation artifacts . Crotamine was able to promote intracellular delivery of the pEGFP-N1 plasmid only into wild-type CHO-K1 cells, demonstrated by the intracellular expression of the associated reporter gene ( …Author: Fábio Dupart Nascimento, Mirian A. F. Hayashi, Alexandre Kerkis, Vitor Oliveira, Eduardo Brandt de O...
https://www.ncbi.nlm.nih.gov/pubmed/17491023
Jul 20, 2007 · Interestingly, crotamine demonstrates cell specificity and targeted delivery of plasmid DNA into actively proliferating cells both in vitro and in vivo, which distinguishes crotamine from other known natural cell-penetrating peptides.Author: Fábio Dupart Nascimento, Mirian A. F. Hayashi, Alexandre Kerkis, Vitor Oliveira, Eduardo Brandt de O...
http://www.jbc.org/content/282/29/21349.short
Jul 20, 2007 · Interestingly, crotamine demonstrates cell specificity and targeted delivery of plasmid DNA into actively proliferating cells both in vitro and in vivo, which distinguishes crotamine from other known natural cell-penetrating peptides. The mechanism of crotamine penetration and cargo delivery into cells was also investigated, showing the involvement of heparan sulfate proteoglycans in the …
https://www.researchgate.net/publication/6340930_Crotamine_Mediates_Gene_Delivery_into_Cells_through_the_Binding_to_Heparan_Sulfate_Proteoglycans
Interestingly, crotamine demonstrates cell specificity and targeted delivery of plasmid DNA into actively proliferating cells both in vitro and in vivo, which distinguishes crotamine from other ...
https://www.nature.com/articles/s41598-019-39842-7
Mar 01, 2019 · In addition to the demonstrated in vitro gene delivery mediated by crotamine in HK-2 cells, the potential applicability of crotamine as prototypic non-viral (gene) delivery nanocarrier to …Author: Joana Darc Campeiro, Wendy Dam, Gabriela Guilherme Monte, Lucas Carvalho Porta, Lilian Caroline Gonç...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3914522/
Crotamine-Targeted Delivery of Plasmid DNA The common feature among the CPPs is their capacity to deliver biologically active molecules into the cells both in vitro and in vivo . The majority of CPPs do not present any cell specificity for the translocation into the cells [ 21 ].Author: Irina Kerkis, Mirian A. F. Hayashi, Alvaro R. B. Prieto da Silva, Alexandre Pereira, Paulo Luiz De S...
https://www.nature.com/articles/3301840
Jan 13, 2003 · For liver gene delivery, both virus-mediated 345 and non-viral systems have been considered. Although some of the virus-mediated gene transfer systems have been found to be quite effective, their usefulness is limited, given that they induce an immune response, leading to the rapid rejection of transduced cells.Author: M A Arangoa, N Düzgüneş, C Tros de Ilarduya
https://www.ncbi.nlm.nih.gov/pubmed/30824773
Mar 01, 2019 · In addition to the demonstrated in vitro gene delivery mediated by crotamine in HK-2 cells, the potential applicability of crotamine as prototypic non-viral (gene) delivery nanocarrier to modulate PTEC gene and/or protein expression was confirmed. PMCID: PMC6397221Author: Joana Darc Campeiro, Wendy Dam, Gabriela Guilherme Monte, Lucas Carvalho Porta, Lilian Caroline Gonç...
https://www.ncbi.nlm.nih.gov/pubmed/12525832
The combination of targeting and protamine condensation obviated the need for partial hepatectomy, commonly required to obtain efficient gene delivery in this organ. Since protamine sulfate has been proven to be non-toxic in humans, the novel liver-specific vector described here may be useful for the delivery of clinically important genes to this organ.Author: M A Arangoa, N Düzgüneş, C Tros de Ilarduya
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