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http://www.biologydiscussion.com/gene/gene-delivery-systems-steps-types/17737
Types of Gene Delivery Systems: Three main types of gene delivery systems have been in use with varying degrees of success with higher plants. These are: (a) Agrobacterium Mediated Gene Transfer (AMGT) System: The bacterium Agrobacterium is known as the Nature’s smallest Genetic Engineer. It is a pathogenic, gram-negative, soil bacterium.
http://www.biologydiscussion.com/genetics/agrobacterium-as-gene-transformation-vector-genetics/71893
The co-integrative vector containing X-gene is then mobilized into Agrobacterium. The disarming of Ti plasmid of Agrobacterium can be accomplished by removing T-DNA except left border. Disarmed Ti plasmid retains vir genes as well as PBR322 homologous sequence.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC150518/
Several excellent reviews have appeared recently that describe in detail various aspects of Agrobacterium biology ( 44, 73, 109, 325, 327, 328, 384, 385 ). In this review, I describe how scientists utilized knowledge of basic Agrobacterium biology to develop Agrobacterium as a “tool” for plant genetic engineering.Author: Stanton B. Gelvin
http://www.biologydiscussion.com/gene/gene-transfer/vectors-of-gene-transfer-genetics/61457
The potential use of the Agrobacterium Ti plasmid (A. tumefaciens) and Ri plasmid (A. rhizogenes) as vectors arises from the ability of the bacterium to somehow transfer and stably integrate a piece of the plasmid DNA into the plant nuclear genome.
https://www.researchgate.net/publication/255958427_Plant_Viral_Vectors_for_Delivery_by_Agrobacterium
Plant viral vectors delivered by Agrobacterium are the basis of several manufacturing processes that are currently in use for producing a wide range of proteins for multiple applications, including vaccine antigens, antibodies, protein nanoparticles such as virus-like particles (VLPs), and other protein and protein-RNA scaffolds.
https://link.springer.com/protocol/10.1007/978-1-4939-7315-6_2
Sep 26, 2017 · Disarmed Agrobacterium strains harboring standard binary vectors and the biolistic gun system Bio-Rad PDS-1000/He are used as gene delivery systems. The herbicide resistant bar gene and selection agent bialaphos are used for identifying putative transgenic type I callus events.Author: Jennifer A. Raji, Bronwyn Frame, Daniel Little, Tri Joko Santoso, Kan Wang
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759027/
Sep 02, 2013 · Our family of vectors include the lacZ α gene cloned between two BsaI or SapI sites, which allows for selection of recombinant clones based on blue-white colony color. The lacZ α gene is flanked by BsaI sites on both sides of the selection marker, and is excised from the vector …Author: Shahram Emami, Muh-ching Yee, José R. Dinneny
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4113218/
The most common methods of gene introduction into plant cells are direct delivery by biolistics or indirect delivery by using Agrobacterium tumefaciens . Biolistics, also known as microprojectile bombardment, involves the coating of gold or tungsten particles (~ 2 microns in diameter) with the transgene and firing them ballistically into plant cells [ 14 ].
http://www.biologydiscussion.com/genetics/engineering/methods-of-gene-transfer-in-plants-2-methods/10824
Agrobacterium-Mediated Gene Transfer: Agrobacterium tumefaciens is a soil-borne, Gram-negative bacterium. It is rod shaped and motile, and belongs to the bacterial family of Rhizobiaceae. A. tumefaciens is a phytopathogen, and is treated as the nature’s most effective plant genetic engineer.
https://www.biologyexams4u.com/2012/12/ti-plasmid-based-vectors-co-integrate.html
Second vector is a disarmed pTi vector = gene of interest+ some markers+pBR 322 sequences. Both intermediate vector and disarmed pTi has some sequences in common (pBR 322 sequences). Therefore by homologous recombination, co-integration of two plasmids will take place within Agrobacterium.
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