Biolistic Delivery

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Biolistic Delivery Systems - Bio-Rad

    http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_5443.pdf
    Particle delivery is a convenient method for transforming these sensitive cells since little pre- or postbombardment manipulation is necessary. Other Biological Systems Biolistic technology has been applied to targets as diverse as chloroplasts, mitochondria, bacteria, fungi, algae, and pollen.File Size: 434KB

Biolistic delivery of Ca2+ dyes into plant and algal cells

    https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-313X.2006.02687.x
    Biolistic delivery of microscopic gold particles coated in DNA is a standard technique used to transform undifferen-tiated plant material (Lorence and Verpoorte, 2004). We show here that particles may also be coated in fluorescent dyes and used to report [Ca2þ] cyt changes in plant and algal cells. This biolistic method is quicker and easier ...Author: John H.F. Bothwell, Colin Brownlee, Alistair M. Hetherington, Carl K.-Y. Ng, Glen L. Wheeler, Martin...

Biolistic Particle Delivery Systems Life Science ...

    https://www.bio-rad.com/en-us/category/biolistic-particle-delivery-systems?ID=09facf6d-b7a7-490a-bb87-9326aa5309e1
    The Helios gene gun is a convenient handheld device that provides direct, rapid, and versatile gene delivery into a range of targets in vivo. PDS-1000 He™ and Hepta™ Systems The PDS-1000/He system uses a burst of high-pressure helium gas to …

Biolistic-delivery-based transient CRISPR/Cas9 expression ...

    https://www.ncbi.nlm.nih.gov/pubmed/30258105
    Sep 26, 2018 · The biolistic delivery of gold particles coated with plasmids expressing CRISPR/Cas9 components designed to target TaGASR7 were bombarded into SAM-exposed embryos of imbibed seeds. Bombarded embryos showing transient GFP expression within SAM were selected and grown into adult plants. Mutations in the target gene were assessed in fifth-leaf ...Author: Haruyasu Hamada, Yuelin Liu, Yozo Nagira, Ryuji Miki, Naoaki Taoka, Ryozo Imai

Biolistics - an overview ScienceDirect Topics

    https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/biolistics
    Biolistic particle delivery or micro-projectile bombardment is a technique by which foreign genes are delivered to cells using heavy metal particles coated with exogenous DNA. The device used for bombardment can act on any type of cell, transforming not only …

Genome editing of bread wheat using biolistic delivery of ...

    https://www.nature.com/articles/nprot.2017.145
    Liang, Z., Chen, K., Zhang, Y. et al. Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 in vitro transcripts or ribonucleoproteins. Nat Protoc 13, …Author: Zhen Liang, Kunling Chen, Yi Zhang, Yi Zhang, Jinxing Liu, Kangquan Yin, Jin-Long Qiu, Caixia Gao

Biolistic Transformation Forage Information System ...

    https://forages.oregonstate.edu/tallfescuemonograph/transgenesis/methods/biolistic
    Biolistic transformation (particle bombardment) is based on the delivery of DNA into plant cells by high velocity gold or tungsten particles (Sanford, 1988). Because DNA delivery by particle bombardment is a physical process that does not depend on bacteria, it has …

Biolistic Transformation - an overview ScienceDirect Topics

    https://www.sciencedirect.com/topics/neuroscience/biolistic-transformation
    Another advantage to using biolistic technology is that a gene gun can deliver DNA through relatively thick tissue, such as a tissue slice in culture. Therefore, it is possible to transfect cells within a slice that would be difficult to target using other gene delivery methods.

A microarray MEMS device for biolistic delivery of vaccine ...

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4635881/
    We report a biolistic technology platform for physical delivery of particle formulations of drugs or vaccines using parallel arrays of microchannels, which generate highly …Author: Fatemeh Nazly Pirmoradi, Ashish V Pattekar, Felicia Linn, Michael I Recht, Armin R Volkel, Qian Wang...

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