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https://www.ncbi.nlm.nih.gov/pubmed/19301647
Bacterial delivery of siRNAs: a new approach to solid tumor therapy. Xu DQ(1), Zhang L, Kopecko DJ, Gao L, Shao Y, Guo B, Zhao L. Author information: (1)Laboratory of Enteric and Sexually Transmitted Diseases, Center for Biologics Evaluation and Research, …Author: De-Qi Xu, Ling Zhang, Dennis J Kopecko, Lifang Gao, Yueting Shao, Baofeng Guo, Lijing Zhao
https://link.springer.com/protocol/10.1007/978-1-60327-547-7_8
Oct 13, 2008 · Bacterial delivery vector Salmonella siRNA shRNA RNAi solid tumor This is a preview of subscription content, log in to check access. Springer Nature is developing a new tool to find and evaluate Protocols.Author: De-Qi Xu, Ling Zhang, Dennis J Kopecko, Lifang Gao, Yueting Shao, Baofeng Guo, Lijing Zhao
https://www.researchgate.net/publication/24215346_Bacterial_Delivery_of_siRNAs_A_New_Approach_to_Solid_Tumor_Therapy
Request PDF Bacterial Delivery of siRNAs: A New Approach to Solid Tumor Therapy RNAi is a powerful research tool for specific gene silencing and may also lead to promising novel therapeutic ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5112363/
Nov 17, 2016 · Delivery of dsRNA using chemically modified molecules, polymer nanoparticles, liposomes, viruses or bacteria, could increase efficacy in attaining a potent RNAi response. The choice of the delivery method and the choice of formulation would of course depend on the circumstances, on the target insect and on the reason for impaired RNAi-efficiency.Author: Mallikarjuna R. Joga, Moises J. Zotti, Guy Smagghe, Olivier Christiaens
https://link.springer.com/protocol/10.1007/978-1-4939-1538-5_7
Sep 23, 2014 · Delivery of siRNAs to Cancer Cells via Bacteria. Abstract. RNA interference (RNAi) technology is a promising approach for efficient silencing of a particular gene for cancer gene therapy. However, the main obstacle for the development of RNAi-based therapeutic approaches is the delivery of the RNAi effector molecules to target cells.Author: Omar Ahmed, Andrea Krühn, Hermann Lage
https://www.nature.com/articles/s12276-019-0297-0
Dec 11, 2019 · However, the largest barrier to RNA interference therapy is the need for specific delivery of siRNAs to the tumor region. Bacteria-based delivery systems for siRNAs against Stat3 63,122,149,152 ...Author: Mai Thi-Quynh Duong, Yeshan Qin, Sung-Hwan You, Jung-Joon Min
https://en.wikipedia.org/wiki/Small_interfering_RNA
siRNAs have a well-defined structure: a short (usually 20 to 24-bp) double-stranded RNA (dsRNA) with phosphorylated 5' ends and hydroxylated 3' ends with two overhanging nucleotides. The Dicer enzyme catalyzes production of siRNAs from long dsRNAs and small hairpin RNAs. siRNAs can also be introduced into cells by transfection.
https://vector.childrenshospital.org/2013/03/rna-interference-putting-bacteria-to-work-to-silence-genes/
Mar 26, 2013 · The bacteria transcribe this foreign DNA to produce a double stranded RNA, which gets chopped into smaller pieces that resemble siRNAs. They call them pro-siRNAs—short for prokaryotic siRNAs ( prokaryote is the scientific name for the group of organisms like bacteria that do not have a …
https://www.sciencedirect.com/science/article/pii/S0024320520302988
Bacterial mediated delivery of foreign DNA into mammalian cells employing bacterial vector is termed as bactofection based gene therapy . Most common species of bacteria used for breast cancer vaccination are Salmonella typhimurium [ 79 ], Listeria monocytogenes [ 80 ], Clostridium novyi [ 81 ], Clostridium acetobutylicum , Bifidobacterium longum , Bifidobacterium adolescentis , and Escherichia coli .Author: Balamurugan Shanmugaraj, Lohanathan Bharathi Priya, B. Mahalakshmi, Shanmugam Subbiah, Rouh-Mei Hu, ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3170882/
Sep 12, 2011 · Whereas delivery of antisense agents into bacteria remains a significant challenge, once delivered, the molecule is surprisingly well retained. These accumulation and retention properties may prove particularly appropriate in new strategies for …Author: Liam Good, James E. M. Stach
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