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https://en.wikipedia.org/wiki/Gene_delivery
Gene delivery is the process of introducing foreign genetic material, such as DNA or RNA, into host cells. Genetic material must reach the nucleus of the host cell to induce gene expression. Successful gene delivery requires the foreign genetic material to remain stable within the host cell and can either integrate into the genome or replicate independently of it.
http://scialert.net/fulltext/?doi=biotech.2008.385.402
DNA-Delivery Methods to Produce Transgenic Plants. Fulltext - DNA-Delivery Methods to Produce Transgenic Plants ... The most common method of delivering foreign DNA into plant protoplasts involves treatment with PEG as a hydrophilic long-chain polymer ... Advances in alternative DNA delivery techniques. Plant Cell Tissue Organ Culture, 40: 1-15.
https://link.springer.com/article/10.1007/s00217-008-0850-x
Mar 11, 2008 · Nucleic acids, especially DNA, are targets of qualitative and quantitative diagnostics for genetically modified organisms (GMO) in seeds, food- and feedstuff. The amplification of the nucleic acid is an essential step for further analyses of the target sequence. The PCR has been the method of choice for DNA amplification in most laboratories, and its real-time version (qPCR) also enables ...Author: Dany Morisset, Dejan Stebih, Katarina Cankar, Jana Zel, Kristina Gruden
https://www.sciencedirect.com/science/article/pii/S0378517313010351
DNA encapsulation using biodegradable polymer is a good alternative to condensing DNA with polycations, and can overcome the obstacles and drawbacks that limit using the condensing strategy to form DNA-protection and delivery systems.Author: D. Ibraheem, A. Elaissari, H. Fessi
https://www.mirusbio.com/transfectopedia/methods
Although microinjection provides a direct method for delivery of nucleic acid for cells that are difficult to transfect, it is low-throughput and a technique that is difficult to master. An alternative and more efficient method for delivering nucleic acid into cells is the use of viral vectors.
https://link.springer.com/article/10.1007%2FBF00041112
Jan 01, 1995 · This review describes recent advances in alternative DNA-delivery techniques with particular emphasis on silicon carbide fibers, intact tissue electroporation, electrophoresis and microinjection. The advantages/disadvantages of each method along with a historical overview and theory of practice are discussed.Author: D. D. Songstad, D. A. Somers, R. J. Griesbach
https://en.wikipedia.org/wiki/Gene_gun
A gene gun is used for delivery of exogenous DNA to cells. This method is known as 'biolistics'. Gene guns can be used effectively on most cells but are mainly used on plant cells. Step 1 The gene gun apparatus is ready to fire. Step 2 Helium fills the chamber and pressure builds against the rupture disk.
http://www.nature.com/articles/nbt0100_33
Jan 01, 2000 · Current synthetic DNA delivery systems are versatile and safe, but substantially less efficient than viruses. ... Another method, ... An alternative approach is to use high-voltage electrical ...Author: Dan Luo, W. M. Saltzman
https://cen.acs.org/articles/96/web/2018/03/Delivering-DNA-tips-nanospears.html
Another gene delivery method involves a kind of cellular shock therapy called electroporation. ... Paul S. Weiss see promise in alternative DNA-delivery strategies, particularly the use of ...
http://agron-www.agron.iastate.edu/ptf/publications/Paz_PCR_2006.pdf
cotyledonary node method using an alternative explant for Agrobacterium tumefaciens-mediated soybean transforma-tion. We use the term “half-seed” to refer to this alternative ... There are two modes of DNA delivery that are currently Communicated by J. C. RegisterAuthor: L. Folling, A. Olesen
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