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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2151968/
Oct 16, 2007 · In conclusion, we have presented a practical approach for protein delivery into live cells and we have demonstrated that the determination of optimal conditions is required for efficient delivery. Routinely, we recommend using 1–2 μg of R-PE and 2–4 μL of …Author: Claire O. Weill, Stéphanie Biri, Abdennaji Adib, Patrick Erbacher
https://www.researchgate.net/publication/23467287_A_practical_approach_for_intracellular_protein_delivery
More importantly, this work offers a robust and safe approach for protein therapeutics and intracellular delivery of other functional peptides, as well as gene-based therapy.
http://europepmc.org/articles/PMC2151968
In conclusion, we have presented a practical approach for protein delivery into live cells and we have demonstrated that the determination of optimal conditions is required for efficient delivery. Routinely, we recommend using 1–2 μg of R-PE and 2–4 μL of delivery reagent to obtain an efficient protein delivery.
https://link.springer.com/article/10.1007/s10616-007-9102-3
Oct 16, 2007 · Protein delivery represents a powerful tool for experiments in live cells including studies of protein-protein interactions, protein interference with blocking antibodies, intracellular trafficking and protein or peptide biological functions. Most available reagents dedicated to the protein delivery allow efficient crossing of the plasma membrane.Author: Claire O. Weill, Stéphanie Biri, Abdennaji Adib, Patrick Erbacher
https://www.deepdyve.com/lp/springer-journals/a-practical-approach-for-intracellular-protein-delivery-n5WMDWwx4s
Oct 16, 2007 · Read "A practical approach for intracellular protein delivery, Cytotechnology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
https://www.onlinelibrary.wiley.com/doi/10.1002/adma.201701086
One‐Pot Synthesis of Multiple Protein‐Encapsulated DNA Flowers and Their Application in Intracellular Protein Delivery. Eunjung Kim. ... , 3 immunostimulatory CpG motif‐containing DNA, 4 and proteins. 5 To enable practical uses of the structures made by DNA ... we report a new approach that directly synthesizes protein‐encapsulated DNA ...Author: Eunjung Kim, Limor Zwi-Dantsis, Natalie Reznikov, Catherine S. Hansel, Shweta Agarwal, Molly M. Stev...
https://www.polyplus-transfection.com/products/pulsin/
The delivery of protein and antibody using PULSin represents a powerful approach for functional studies.For example, PULSin enables you to study lethal proteins by controlling the level and time course of protein delivery into the cells.Cell types: Adherent & suspension cells grown in presence of serum
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6207587/
Oct 24, 2018 · We hope that both the elucidation of normal behavior of antibodies within cells, including their relocation and degradation pathways, as well as the development of specific, effective, and clinically applicable systems of targeted intracellular protein delivery will allow this approach to be used as a clinical therapy in future.Author: Tatiana A. Slastnikova, A. V. Ulasov, A. A. Rosenkranz, A. A. Rosenkranz, A. S. Sobolev, A. S. Sobol...
https://pubs.acs.org/doi/10.1021/acsami.8b21724
Delivery of proteins to carry out desired biological functions is a direct approach for disease treatment. However, protein therapy is still facing challenges due to low delivery efficiency, poor targeting during trafficking, insufficient therapeutic efficacy, and possible toxicity induced by carriers. Here, we present a novel delivery platform based on DNA origami nanostructure that enables ...Author: Shuai Zhao, Shuai Zhao, Fangyuan Duan, Shaoli Liu, Shaoli Liu, Tiantian Wu, Tiantian Wu, Yingxu Shan...
https://www.researchgate.net/publication/264502458_Proteolistics_A_biolistic_method_for_intracellular_delivery_of_proteins
In this work, an intracellular protein delivery methodology termed "proteolistics" is described. This method utilizes a biolistic gun apparatus and involves a simple protein/projectile preparation ...
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